FPR1 Antibody

Images

 
Western Blot: FPR1 Antibody [NB100-56473] - Analysis of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using this antibody at 1 ug/ml.
Simple Western: FPR1 Antibody [NB100-56473] - Shows a specific band for FPR1 in 0.5 mg/ml of Mouse Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FPR1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 176-190 of human FPR1 (CTFNFSPWTNDPKER) was used as the immunogen
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FPR1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 100 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-56473.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FPR1 Antibody

  • fMet-Leu-Phe receptor
  • fMLF-R
  • fMLP receptor
  • FMLP
  • formyl peptide receptor 1
  • FPR1
  • FPRfMet-Leu-Phe receptor
  • N-formyl peptide receptor
  • N-formylpeptide chemoattractant receptor

Background

FPR1 is a member of chemoattractant/chemokine GPCRs expressed mainly on leukocytes, neutrophils and monocytes. FPR1 is a seven transmembrane pertussis toxin-sensitive GPCR and binds to formylated peptides generated by bacteria resulting in leukocyte trafficking to the site of inflammation. Ligand binding to FPR results in activation of multiple effectors including phospholipase C leading to calcium mobilization and PKC activation. Other implications of FPR signaling include polarization of actin cytoskeleton, activation of various integrins, increased cell migration, phagocytosis, degranulation and release of reactive oxygen intermediates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FPR1 Antibody (NB100-56473)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-56473 Applications Species
Zayas JP Molecular Dissection of Impaired Infection Control in Diabetic Wound Thesis Jan 1 2020

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol FPR1
Entrez
Uniprot