FMO3 Antibody - BSA Free

Immunohistochemistry-Paraffin: FMO3 Antibody [NBP1-86083] - Analysis in human liver and testis tissues using NBP1-86083 antibody. Corresponding FMO3 RNA-seq data are presented for the same tissues.

Staining of human endometrium shows no positivity in glandular cells.

Staining of human small intestine shows very weak positivity in glandular cells.

Staining of human liver shows strong cytoplasmic and membranous positivity in hepatocytes.

Staining of human testis shows very weak positivity in Leydig cells.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FMO3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FMO3 Antibody - BSA Free (NBP1-86083) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IYKSVFSNSSKEMMCFPDFPFPDDFPNFMHNSKIQEYIIAFAKEKNLLKYIQFKTFVSSVNKHPDFATTGQWDVTTERDGKKESAVFDAVMVCSGHHVYPNLPK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FMO3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  FMO3 Protein (NBP1-86083PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for FMO3 Antibody - BSA Free

• dimethylaniline monooxygenase [N-oxide-forming] 3
• Dimethylaniline oxidase 3
• dJ127D3.1
• EC 1.14.13.8
• flavin containing monooxygenase 3
• FMO 3
• FMO form 2
• FMO II
• FMOII
• Hepatic flavin-containing monooxygenase 3
• hepatic flavin-containing monooxygenase-3
• MGC34400
• TMAU

Background

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FMO3