FMO3 Antibody

Images

 
Western Blot: FMO3 Antibody [NBP1-33583] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FMO3 Antibody [NBP1-33583] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: FMO3 Antibody [NBP1-33583] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.
Western Blot: FMO3 Antibody [NBP1-33583] - Sample (50 ug of whole cell lysate) A: Mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.91 mg/ml

Order Details

FMO3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FMO3. The exact sequence is proprietary.
Localization
Microsome membrane, Endoplasmic reticulum membrane
Predicted Species
Primate (100%), Rhesus Macaque (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FMO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Rat (81%), Canine (84%), Rabbit (81%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.91 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FMO3 Antibody

  • dimethylaniline monooxygenase [N-oxide-forming] 3
  • Dimethylaniline oxidase 3
  • dJ127D3.1
  • EC 1.14.13.8
  • flavin containing monooxygenase 3
  • FMO 3
  • FMO form 2
  • FMO II
  • FMOII
  • Hepatic flavin-containing monooxygenase 3
  • hepatic flavin-containing monooxygenase-3
  • MGC34400
  • TMAU

Background

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FMO3