FMO3 Antibody (JE55-13)

Western Blot: FMO3 Antibody (JE55-13) [NBP3-32344] - Western blot analysis of FMO3 on different lysates with Rabbit anti-FMO3 antibody (NBP3-32344) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat liver tissue lysate Lane 5: Rat kidney tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32344) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: FMO3 Antibody (JE55-13) [NBP3-32344] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-FMO3 antibody (NBP3-32344) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32344) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: JE55-13
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

FMO3 Antibody (JE55-13) Summary

• Description: Novus Biologicals Rabbit FMO3 Antibody (JE55-13) (NBP3-32344) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human FMO3 aa 281-380 / 532. (Uniprot: P31513)
• Localization: Microsome membrane, Endoplasmic reticulum membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FMO3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:1000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FMO3 Antibody (JE55-13)

• dimethylaniline monooxygenase [N-oxide-forming] 3
• Dimethylaniline oxidase 3
• dJ127D3.1
• EC 1.14.13.8
• flavin containing monooxygenase 3
• FMO 3
• FMO form 2
• FMO II
• FMOII
• Hepatic flavin-containing monooxygenase 3
• hepatic flavin-containing monooxygenase-3
• MGC34400
• TMAU

Background

The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme (Ziegler, 1990 [PubMed 2203193]; Hines et al., 1994 [PubMed 8128486]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FMO3