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Flt-3/Flk-2/CD135 Antibody (A2F10)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IP
Clone
A2F10
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43352AF350
Alexa Fluor 405
NBP1-43352AF405
Alexa Fluor 488
NBP1-43352AF488
Alexa Fluor 532
NBP1-43352AF532
Alexa Fluor 594
NBP1-43352AF594
Alexa Fluor 647
NBP1-43352AF647
Alexa Fluor 700
NBP1-43352AF700
Alexa Fluor 750
NBP1-43352AF750
Allophycocyanin
NBP1-43352APC
Allophycocyanin/Cy7
NBP1-43352APCCY7
Biotin
NBP1-43582-0.1mg
Biotin
NBP1-43582-0.05mg
Biotin
NBP1-43582-0.5mg
DyLight 350
NBP1-43352UV
DyLight 405
NBP1-43352V
DyLight 488
NBP1-43352G
DyLight 550
NBP1-43352R
DyLight 594
NBP1-43352DL594
DyLight 650
NBP1-43352C
DyLight 680
NBP1-43352FR
DyLight 755
NBP1-43352IR
Janelia Fluor 549
NBP1-43352JF549
Janelia Fluor 646
NBP1-43352JF646
PE
NBP1-43352PE
PE/Atto594
NBP1-43352PEATT594
PE/Cy5
NBP1-43078-0.1mg
PE/Cy5
NBP1-43078-0.05mg
PE/Cy5.5
NBP1-43352PECY55
PE/Cy7
NBP1-43352PECY7
PerCP
NBP1-43352PCP
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Flt-3/Flk-2/CD135 Antibody (A2F10) Summary

Immunogen
The immunogen for this antibody was CD135.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
FLT3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry < = 0.5 ug/10^6 cells in 100 ul
  • Immunoprecipitation 1:10-1:500
Application Notes
Product has also been reported in blocking of Flk-2/Flt3 ligand in functional studies.
Publications
Read Publication using
NBP1-43352 in the following applications:

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Flt-3/Flk-2/CD135 Antibody (A2F10)

  • CD135 antigen
  • CD135
  • EC 2.7.10
  • fetal liver kinase 2
  • FL cytokine receptor
  • FLK2
  • Flk-2
  • FLT3 receptor tyrosine kinase
  • Flt3
  • Flt-3
  • Fms-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • growth factor receptor tyrosine kinase type III
  • Stem cell tyrosine kinase 1
  • STK-1
  • STK1EC 2.7.10.1
  • Tyrosine-protein kinase receptor FLT3

Background

The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and or reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).
Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Flt-3 Ligand
[Unconjugated]
308-FK
1&amp;nbsp;µg/lane of Recombinant Human Flt-3 Ligand was resolved with SDS-PAGE under&amp;nbsp;reducing (R) conditions and visualized&amp;nbsp;by&amp;nbsp;silver staining, showing major bands at 22‑26&amp;nbsp;kDa. The multiple bands are due to variable glycosylation of the protein.
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CD117/c-kit
Antibody [Unconjugate
AF1356
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1&amp;nbsp;µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;
(9 Reviews)
(30 Publications)
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Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
SCF/c-kit Ligand
[Unconjugated]
7466-SC/CF
SCF/c-kit Ligand [Unconjugated]
(3 Publications)
Species: Hu
CD34 Antibody (MEC
14.7)
NB600-1071
Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
(19 Publications)
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
IL-3 [Unconjugated]
203-IL/CF
1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-3 (Catalog #203‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02‑0.1&amp;nbsp;ng/mL.
(131 Publications)
Species: Hu
STAT5a/b Antibody
[Unconjugated] - Pan ...
AF2168
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). Specific bands were detected for STAT5a at approximately 94&amp;nbsp;kDa and STAT5b at approximately 92&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL‑3 (Catalog&amp;nbsp;# 403-ML) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT5a/b/DNA complexes were immunoprecipitated using 5 μg Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) or control antibody (Catalog&amp;nbsp;# AB-105-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# BAF008). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog&amp;nbsp;# MAG999) and DNA was purified using chelating resin solution. The bcl-x promoter was detected by standard PCR.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for STAT5a/b at approximately 91&amp;nbsp;kDa (as indicated) using 20&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
(9 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ChIP
STAT5b Antibody
[Unconjugated]
AF1584
HeLa human cervical epithelial carcinoma and M1 mouse myeloid leukemia cell line were untreated (-) or activated (+) with 100 ng/mL Recombinant Human IFN‑ gamma (Catalog&amp;nbsp;# 285-IF) for 15&amp;nbsp;minutes. STAT5b was immunoprecipitated from lysates of 5 x 106 cells following incubation with 2&amp;nbsp;µg&amp;nbsp;Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584) for 1 hour at room temperature. STAT5b-antibody complexes were absorbed using Protein A sepharose (Invitrogen, Catalog # 10-1041). Immunoprecipitated STAT5b was detected by Western blot using 1&amp;nbsp;µg/mL Human Phospho-STAT5a/b (Y699) Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF4190). View our recommended buffer recipes for immunoprecipitation.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, M1 mouse myeloid leukemia cell line, and&amp;nbsp;PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). A specific band was detected for STAT5b at approximately 90&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Jak2 [p Tyr1007, p
Tyr1008] Antibody (SY ...
NBP2-67429
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IL-6 [Unconjugated]
7270-IL/CF
IL-6 [Unconjugated]
Species: Hu
CEBP alpha
Antibody (15C8)
NB600-1438
Western Blot: CEBP alpha Antibody (15C8) [NB600-1438] - Analysis of transfected 293T cells.
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MID1 Antibody
NBP1-26612
Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.
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CTLA-4
[Unconjugated]
7268-CT
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NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Immunohistochemistry-Paraffin: M-CSF R/CD115 Antibody (6B9H2) [NBP2-37289] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CSF1R mouse mAb with DAB staining.
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HLA DQ Antibody
(SPV-L3)
NBP2-45041
HLA DQ Antibody (SPV-L3)
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Applications: Flow, IHC-Fr, IF
IL-7 [Unconjugated]
207-IL
IL-7 [Unconjugated]
(6 Reviews)
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100&amp;nbsp;ng/mL Human&amp;nbsp;PDGF&amp;nbsp;(Catalog&amp;nbsp;# 120‑HD) for 15&amp;nbsp;minutes and MCF‑7 human breast cancer cell line untreated or treated with 100&amp;nbsp;ng/mL&amp;nbsp;Recombinant Human IGF-1 (Catalog&amp;nbsp;# 291‑G1) for 15&amp;nbsp;minutes. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887) at 10&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog&amp;nbsp;# 291-G1) for 15&amp;nbsp;minutes, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
(21 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow

Publications for Flt-3/Flk-2/CD135 Antibody (NBP1-43352)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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FAQs for Flt-3/Flk-2/CD135 Antibody (NBP1-43352) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43352AF350
Alexa Fluor 405 NBP1-43352AF405
Alexa Fluor 488 NBP1-43352AF488
Alexa Fluor 532 NBP1-43352AF532
Alexa Fluor 594 NBP1-43352AF594
Alexa Fluor 647 NBP1-43352AF647
Alexa Fluor 700 NBP1-43352AF700
Alexa Fluor 750 NBP1-43352AF750
Allophycocyanin NBP1-43352APC
Allophycocyanin/Cy7 NBP1-43352APCCY7
Biotin NBP1-43582
Biotin NBP1-43582
Biotin NBP1-43582
DyLight 350 NBP1-43352UV
DyLight 405 NBP1-43352V
DyLight 488 NBP1-43352G
DyLight 550 NBP1-43352R
DyLight 594 NBP1-43352DL594
DyLight 650 NBP1-43352C
DyLight 680 NBP1-43352FR
DyLight 755 NBP1-43352IR
Janelia Fluor 549 NBP1-43352JF549
Janelia Fluor 646 NBP1-43352JF646
PE NBP1-43352PE
PE/Atto594 NBP1-43352PEATT594
PE/Cy5 NBP1-43078
PE/Cy5 NBP1-43078
PE/Cy5.5 NBP1-43352PECY55
PE/Cy7 NBP1-43352PECY7
PerCP NBP1-43352PCP
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Diseases for Flt-3/Flk-2/CD135 Antibody (NBP1-43352)

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Bioinformatics

Gene Symbol FLT3