Flt-3/Flk-2/CD135 Antibody (A2F10) - BSA Free

• Reactivity: Hu, Mu
• Applications: Flow, IP
• Clone: A2F10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Flt-3/Flk-2/CD135 Antibody (A2F10) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD135.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: FLT3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.5 ug/10^6 cells in 100 ul
  • Immunoprecipitation 1:10-1:500
• Application Notes: Product has also been reported in blocking of Flk-2/Flt3 ligand in functional studies.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Flt-3/Flk-2/CD135 Antibody (A2F10) - BSA Free

• CD135 antigen
• CD135
• EC 2.7.10
• fetal liver kinase 2
• FL cytokine receptor
• FLK2
• Flk-2
• FLT3 receptor tyrosine kinase
• Flt3
• Flt-3
• Fms-like tyrosine kinase 3
• fms-related tyrosine kinase 3
• growth factor receptor tyrosine kinase type III
• Stem cell tyrosine kinase 1
• STK-1
• STK1EC 2.7.10.1
• Tyrosine-protein kinase receptor FLT3

Background

The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and or reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3/Flk-2/CD135 Antibody (NBP1-43352)(3)

Publications using NBP1-43352

• Morales-Mantilla DE, Kain B, Le D Et al. Hematopoietic stem and progenitor cells improve survival from sepsis by boosting immunomodulatory cells Elife 2022-02-15 [PMID: 35166205]
  
  Details:
  Citation using the DyLight 680 version of this antibody.
• Hopner SS, Raykova A, Radpour R et al. LIGHT/LT beta R signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells Nature communications 2021-02-16 [PMID: 33594067] (FLOW)
• Caldarelli A, Muller JP, Paskowski-Rogacz M et al. A genome-wide RNAi screen identifies proteins modulating aberrant FLT3-ITD signaling. Leukemia 2013-03-19 [PMID: 23508117] (FLOW, Mouse)
  
  Details:
  Using the PE conjugated version of NBP1-43352, catalog number NBP1-43352PE.

Bioinformatics

• Gene Symbol: FLT3