Flt-3/Flk-2/CD135 Antibody (A2F10) [PE]

• Reactivity: Hu, Mu
• Applications: Flow, IP
• Clone: A2F10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PE

Flt-3/Flk-2/CD135 Antibody (A2F10) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: The immunogen for this antibody was CD135.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: FLT3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-43352. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Flt-3/Flk-2/CD135 Antibody (A2F10) [PE]

• CD135 antigen
• CD135
• EC 2.7.10
• fetal liver kinase 2
• FL cytokine receptor
• FLK2
• Flk-2
• FLT3 receptor tyrosine kinase
• Flt3
• Flt-3
• Fms-like tyrosine kinase 3
• fms-related tyrosine kinase 3
• growth factor receptor tyrosine kinase type III
• Stem cell tyrosine kinase 1
• STK-1
• STK1EC 2.7.10.1
• Tyrosine-protein kinase receptor FLT3

Background

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3/Flk-2/CD135 Antibody (NBP1-43352PE)(1)

Publication using NBP1-43352PE

• Caldarelli A, Muller JP, Paskowski-Rogacz M et al. A genome-wide RNAi screen identifies proteins modulating aberrant FLT3-ITD signaling. Leukemia 2013-03-19 [PMID: 23508117] (FLOW, Mouse)
  
  Details:
  Using the PE conjugated version of NBP1-43352, catalog number NBP1-43352PE.

Bioinformatics

• Gene Symbol: FLT3