FLI1 Antibody (FLI1/4371R)

Western blot analysis of Daudi cell lysate using FLI1 antibody (FLI1/4371R).

Analysis of Protein Array containing more than 19,000 full-length human proteins using Fli1 Recombinant Rabbit Monoclonal Antibody (FLI1/4371R).

Western blot analysis of Daudi cell lysate using FLI1 antibody (FLI1/4371R).

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma (EWS) stained with FLI1 antibody (FLI1/4371R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma (EWS) stained with FLI1 antibody (FLI1/4371R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: FLI1/4371R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

FLI1 Antibody (FLI1/4371R) Summary

• Description: Positive Controls: THP1 or Raji cells. Human tonsil, Ewing s sarcoma (EWS) or angiosarcoma.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human FLI1 protein
• Localization: Nucleus.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FLI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for FLI1 Antibody (FLI1/4371R)

• ERGB
• EWSR2
• FLI1
• Friend leukemia integration 1 transcription factor
• Friend leukemia virus integration 1
• Proto-oncogene Fli-1
• SIC-1
• Transcription factor ERGB

Background

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLI1