Ficolin-1 Antibody (7G1)

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 7G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Ficolin-1 Antibody (7G1) Summary

• Description: Novus Biologicals Mouse Ficolin-1 Antibody (7G1) (NBP3-11282) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length recombinant Ficolin-1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoassay
  • Western Blot
• Application Notes: For Immunoassays and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Ficolin-1 Antibody (7G1)

• Collagen/fibrinogen domain-containing protein 1
• FCN1
• Fcna
• FCNM
• FCNMficolin-A
• ficolin (collagen/fibrinogen domain containing) 1
• ficolin (collagen/fibrinogen domain-containing) 1
• Ficolin1
• Ficolin-1
• Ficolin-A
• ficolin-alpha
• M-ficolin

Background

The monoclonal antibody 7G1 recognizes human M-ficolin, also known as ficolin-1. Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: M-Ficolin, L- Ficolin (Ficolin-2), and H-Ficolin (Ficolin-3). M-Ficolin, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. However, it was recently also found in serum suggesting that M-Ficolin also has a role in systemic immunity like L- and H-Ficolin. Functional analysis of M-Ficolin has demonstrated that it's ligand is sialic acid present on bacteria since binding was abolished by prior treatment of the bacteria with sialidase. Furthermore, it was shown that M-ficolin facilitates the uptake of E. coli- by a leukemic monocyte lymphoma cell line, suggesting that it functions as a phagocytic receptor on the surface of circulating monocytes. Low cord blood concentration of M-ficolin has been- associated with higher necrotizing enterocolitis-associated fatality and two identified SNPs in the M-ficolin gene are associated with the development of rheumatoid arthritis. The monoclonal antibody 7G1 is suitable for use in immunoassays and western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCN1