FGF basic/FGF2/bFGF Antibody [Unconjugated]

Images

 
Bovine FGF basic/FGF2/bFGF (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic/FGF2/bFGF/bFGF ...read more

Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

FGF basic/FGF2/bFGF Antibody [Unconjugated] Summary

Immunogen
Bovine brain-derived FGF basic/FGF2/bFGF
Specificity
Detects bovine FGF basic/FGF2/bFGF in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with FGF acidic and recombinant human (rh)  beta -ECGF is observed and less than 1% cross-reactivity with rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, recombinant mouse (rm) FGF-8b, rmFGF-8c, rhFGF-9, rhFGF-10, rmFGF-15, rhFGF-17, and rhFGF-18 is observed. Neutralizes the biological activity of bovine FGF basic/FGF2/bFGF and will also neutralize the biological activity of recombinant human FGF basic/FGF2/bFGF.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FGF2
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 1.5-4.5 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AB-33-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF basic/FGF2/bFGF Antibody [Unconjugated]

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Background

FGF basic is a member of the FGF family, currently comprised of seven related mitogenic proteins which show 35‑55% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF basic in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities.

FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF basic/FGF2/bFGF Antibody (AB-33-NA)(5)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Ovine.

We have publications tested in 4 applications: Functional Assay, IHC-P, Neutralization, Western Blot.


Filter By Application
Functional Assay
(1)
IHC-P
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Bovine
(1)
Ovine
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using AB-33-NA Applications Species
RJ Lehmann, LA Jolly, BV Johnson, MS Lord, HN Kim, JT Saville, M Fuller, S Byers, ALK Derrick-Ro Impaired neural differentiation of MPS IIIA patient induced pluripotent stem cell-derived neural progenitor cells Molecular genetics and metabolism reports, 2021;29(0):100811. 2021 [PMID: 34712574] (Functional Assay, Human) Functional Assay Human
Ma Y, Zhu MJ, Zhang L, Hein SM, Nathanielsz PW, Ford SP Maternal obesity and overnutrition alter fetal growth rate and cotyledonary vascularity and angiogenic factor expression in the ewe. Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010;299(1):R249-58. 2010 [PMID: 20427725] (IHC-P, Ovine) IHC-P Ovine
Lu J, Yang JH, Burns AR, Chen HH, Tang D, Walterscheid JP, Suzuki S, Yang CY, Sawamura T, Chen CH Mediation of electronegative low-density lipoprotein signaling by LOX-1: a possible mechanism of endothelial apoptosis. Circ. Res., 2009;104(5):619-27. 2009 [PMID: 19150883] (Western Blot, Bovine) Western Blot Bovine
Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL Dedicated epithelial recipient cells determine pigmentation patterns. Cell, 2007;130(5):932-42. 2007 [PMID: 17803914] (Neutralization, Mouse) Neutralization Mouse
Viti J, Gulacsi A, Lillien L Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2. J. Neurosci., 2003;23(13):5919-27. 2003 [PMID: 12843296] (Neutralization, Mouse) Neutralization Mouse

Reviews for FGF basic/FGF2/bFGF Antibody (AB-33-NA) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGF basic/FGF2/bFGF Antibody (AB-33-NA). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FGF2