FGF-8 Antibody (47109)

FGF‑8 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse FGF‑8 Monoclonal Antibody (Catalog # MAB323) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Mouse FGF‑8 c Isoform (Catalog # 424-FC) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF‑8 c Isoform (125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse FGF‑8 Monoclonal Antibody (Catalog # MAB323). The ND50 is typically 0.25-0.75 µg/mL in the presence of heparin (0.1 µg/mL).

• Reactivity: Hu, Mu
• Applications: WB, IHC, B/N
• Clone: 47109
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGF-8 Antibody (47109) Summary

• Immunogen: E. coli-derived recombinant mouse FGF-8b
  Gln23-Arg215
  Accession # NP_001159834
• Specificity: Detects human and mouse FGF-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) FGF-5, rhFGF-7, and rhFGF-9 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF8
• Purity: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.25-0.75 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-8 Antibody (47109)

• AIGF
• AIGFKAL6
• Androgen-induced growth factor
• FGF8
• FGF-8
• fibroblast growth factor 8 (androgen-induced)
• fibroblast growth factor 8
• HBGF-8
• Heparin-binding growth factor 8
• MGC149376

Background

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
4. Gemel, J. et al. (1996) Genomics 35:253.
5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
6. Crossley, P.H. et al. (1996) Cell, 84:127.
7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
8. Sun, X. et al. (1999) Genes Dev. 13:1834.
9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-8 Antibody (MAB323)(6)

Publications using MAB323

• P Nowialis, K Lopusna, J Opavska, SL Haney, A Abraham, P Sheng, A Riva, A Natarajan, O Guryanova, M Simpson, R Hlady, M Xie, R Opavsky Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions Nat Commun, 2019;10(1):4374. 2019 [PMID: 31558711] (Western Blot, Mouse)
• JM Bae, JC Clarke, H Rashid, MD Adhami, K McCullough, JS Scott, H Chen, KM Sinha, B de Crombru, A Javed Specificity protein 7 is required for proliferation and differentiation of ameloblasts and odontoblasts J. Bone Miner. Res., 2018;0(0):. 2018 [PMID: 29405385] (Western Blot, Mouse)
• Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: Effects on Embryo Survival J Biol Chem, 2016;0(0):. 2016 [PMID: 27402846] (Western Blot, Human)
• Nonomura K, Yamaguchi Y, Hamachi M, Koike M, Uchiyama Y, Nakazato K, Mochizuki A, Sakaue-Sawano A, Miyawaki A, Yoshida H, Kuida K, Miura M Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells. Dev Cell, 2013;27(6):621-34. 2013 [PMID: 24369835] (IHC, Mouse)
• Wang C, Xia C, Bian W, Liu L, Lin W, Chen YG, Ang SL, Jing N Cell aggregation-induced FGF8 elevation is essential for P19 cell neural differentiation. Mol. Biol. Cell, 2006;17(7):3075-84. 2006 [PMID: 16641368] (ICC, Mouse)
• Alsan BH, 2019, Schultheiss TM Regulation of avian cardiogenesis by Fgf8 signaling. Development, 2002;129(8):1935-43. 2002 [PMID: 11934859] (In Ovo, Chicken)

Bioinformatics

• Gene Symbol: FGF8
• Uniprot:
  • NP_001159834()