FGF-8 Antibody (47109) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu
• Clone: 47109
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

FGF-8 Antibody (47109) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human and mouse FGF-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) FGF-5, rhFGF-7, and rhFGF-9 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-8 Antibody (47109) [Alexa Fluor™ Plus 647]

• AIGF
• AIGFKAL6
• Androgen-induced growth factor
• FGF8
• FGF-8
• fibroblast growth factor 8 (androgen-induced)
• fibroblast growth factor 8
• HBGF-8
• Heparin-binding growth factor 8
• MGC149376

Background

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
4. Gemel, J. et al. (1996) Genomics 35:253.
5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
6. Crossley, P.H. et al. (1996) Cell, 84:127.
7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
8. Sun, X. et al. (1999) Genes Dev. 13:1834.
9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.