Recombinant Human FGF-4 Protein Summary
A single, non-glycosylated polypeptide chain corresponding to 182 residues of FGF4.
Source: E. coli
Amino Acid Sequence: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
Fibroblast Growth Factor 4 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
| Protein/Peptide Type
>96%, by HPLC
Less than 1 EU/ug of rHuFGF-4 as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using
NBP2-34864 in the following application:
Packaging, Storage & Formulations
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4, 300 mM NaCl.
>96%, by HPLC
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Alternate Names for Recombinant Human FGF-4 Protein
- fibroblast growth factor 4
- HBGF-4Transforming protein KS3
- heparin secretory transforming protein 1
- Heparin secretory-transforming protein 1
- Heparin-binding growth factor 4
- HSTF1fibroblast growth factor 4 splice isoform
- human stomach cancer, transforming factor from FGF-related oncogene
- kaposi sarcoma oncogene
- oncogene HST
FGF4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF4 shares about 30 % sequence identity with the prototypical members of the FGF family.)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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Review for FGF-4 Protein (NBP2-34864) (1)
Average Rating: 5
(Based on 1 review)
Reviews using NBP2-34864:
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|Comments||I ranked this protein with 5 stars because the cell differentiation was successfully performed.|
|Comments||SDS PAGE gel with Coomassie blue staining showing detection of Human FGF-4 Protein (NBP2-34864-25 ug) (3 ug).|
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