Recombinant Human FGF-4 Protein Summary
A biologically active protein to FGF4.
Source: E. coli
Amino Acid Sequence: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
Fibroblast Growth Factor 4 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 106 IU/mg
| Protein/Peptide Type
Biologically Active Protein
>96%, by HPLC
Less than 1 EU/ug of endotoxin as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using
NBP2-34864 in the following application:
Packaging, Storage & Formulations
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4, 300 mM NaCl.
>96%, by HPLC
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human FGF-4 Protein
- fibroblast growth factor 4
- HBGF-4Transforming protein KS3
- heparin secretory transforming protein 1
- Heparin secretory-transforming protein 1
- Heparin-binding growth factor 4
- HSTF1fibroblast growth factor 4 splice isoform
- human stomach cancer, transforming factor from FGF-related oncogene
- kaposi sarcoma oncogene
- oncogene HST
FGF4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF4 shares about 30 % sequence identity with the prototypical members of the FGF family.)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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Publications for FGF-4 Protein (NBP2-34864) (0)
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Review for FGF-4 Protein (NBP2-34864) (1)
Average Rating: 5
(Based on 1 review)
Reviews using NBP2-34864:
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|Comments||I ranked this protein with 5 stars because the cell differentiation was successfully performed.|
|Comments||SDS PAGE gel with Coomassie blue staining showing detection of Human FGF-4 Protein (NBP2-34864-25 ug) (3 ug).|
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