Fetuin Antibody - BSA Free

Western Blot: Fetuin Antibody [NBP2-16470] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Fetuin Antibody [NBP2-16470] - Fetuin antibody detects Fetuin protein at cytoplasm by immunofluorescent analysis. Sample: SK-N_SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Fetuin protein stained by Fetuin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Fetuin Antibody [NBP2-16470] - Analysis of paraffin-embedded Hepatoma, using antibody at 1:100 dilution.

Western Blot: Fetuin Antibody [NBP2-16470] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: Fetuin Antibody [NBP2-16470] - Sample (50 ug of whole cell lysate) A: Mouse Kidney, 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: Fetuin Antibody [NBP2-16470] - Fetuin antibody detects Fetuin protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin antibody diluted by 1:1000.

Western Blot: Fetuin Antibody [NBP2-16470] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunoprecipitation: Fetuin Antibody [NBP2-16470] - Immunoprecipitation of Fetuin protein from SK-N-SH whole cell extracts using 5 ug of Fetuin antibody. Western blot analysis was performed using Fetuin antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Fetuin Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Fetuin. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FETUB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Fetuin Antibody - BSA Free

• fetuin B
• fetuin-B
• Fetuin-like protein IRL685,16G2
• Gugu
• IRL685

Background

The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FETUB
• Uniprot:
  • Q9UGM5()