FCRLA/FCRLM1 Antibody (865910) [Unconjugated]

Images

 
Western blot shows lysates of CH‑1 mouse B cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse FCRLA Monoclonal Antibody (Catalog # MAB7905) followed by HRP-conjugated Anti-Rat IgG ...read more
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse FCRLA Monoclonal Antibody (Catalog # MAB7905) or (B) Rat IgG2A Isotype ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
865910
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

FCRLA/FCRLM1 Antibody (865910) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse FCRLA
Lys221-Lys352
Accession # Q920A9
Specificity
Detects mouse FCRLA in ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FCRLA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRLA/FCRLM1 Antibody (865910) [Unconjugated]

  • Fc receptor homolog expressed in B cells (FREB)
  • Fc receptor homolog expressed in B-cells
  • Fc receptor related protein X
  • Fc receptor-like A
  • Fc receptor-like and mucin-like 1
  • Fc receptor-related protein X
  • FCRL1
  • FCRLA
  • FCRLb
  • FCRLc1
  • FCRLc2
  • FCRLd
  • FCRLe
  • FCRLFc receptor-like protein
  • FCRLM1
  • FCRLX
  • FcRX
  • FREB
  • FREBFc receptor-like and mucin-like protein 1
  • MGC4595

Background

FCRLA (Fc Receptor-Like A; also known as FcRX and Fc receptor Homolog Expressed in B cells) is a 44 kDa intracellular member of the Fc gamma RI class, FcR family, Immunoglobulin Superfamily of molecules. It is associated with B lineage cells, and has been identified in virtually all splenic B cells, peritoneal B1b and B2 B cells, and immature through mature bone marrow B cells. Not all cells show equal expression patterns. In the spleen, weak FCRLA expression occurs in naïve follicular and marginal zone B cells, and increases with activation state. FcRLA is suggested to act as an ER chaperone during antibody maturation, and is known to bind to IgM, IgA, and IgG prior to their secretion. Mouse FCRLA is synthesized as a 34 kDa, 322 amino acid (aa) precursor that contains a 30 aa signal sequence, two C2‑type Ig‑like domains (aa 80-169 and 182-26), and a C-terminal poly-Proline region (aa 289-294). Although there is no traditional ER retention signal, a viable substitute is assumed to exist at the N-terminus of the mature molecule. FCRLA is believed to exist naturally as a monomer; however, disulfide-linkage can occur during experimental manipulation. While four potential isoform variants are reported, it is unclear if any are actually expressed. One shows an Ala insertion after Ala28, a second shows an AlaIle substitution for aa 20-22, a third contains that previous AlaIle substitution coupled to a deletion of aa 90-115, while a fourth utilizes an alternative start site at Met62. Over aa 221-352, mouse FCRLA shares 68% and 55% aa sequence identity with rat and human FCRLA, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FCRLA
Uniprot