FCRL5/FcRH5/IRTA2 Antibody

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CD19+ human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Goat Anti-Human FCRL5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2087, filled histogram) or isotype control antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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FCRL5/FcRH5/IRTA2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human FCRL5/FcRH5
Gln16-Arg844
Accession # AAK93971
Specificity
Detects human FCRL5/FcRH5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) IRTA3, rhIRTA4, and rhIRTA5 is observed and less than 5% cross-reactivity with rhIRTA1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCRL5
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2087 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRL5/FcRH5/IRTA2 Antibody

  • BXMAS1
  • BXMAS1MGC119592
  • CD307
  • CD307e antigen
  • CD307e
  • DKFZp667E2019
  • DKFZp667F216
  • Fc receptor homolog 5
  • Fc receptor-like 5
  • Fc receptor-like protein 5
  • FcRH5
  • FCRH5MGC119593
  • FCRL5
  • FcR-like protein 5
  • FLJ00333
  • FLJ00397
  • IFGP5
  • Immune receptor translocation-associated protein 2
  • immunoglobulin superfamily receptor translocation associated 2 (IRTA2)
  • IRTA2
  • IRTA2MGC119590
  • PRO820

Background

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5‑7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up‑regulated on tumor cells in some types of B cell malignancies (6, 10‑12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. SwissProt # Q96RD9.
  5. Miller, I. et al. (2002) Blood 99:2662.
  6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
  8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
  9. Mohan, J. et al. (2006) Blood 107:4433.
  10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
  11. Ise, T. et al. (2007) Leukemia 21:169.
  12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
  13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for FCRL5/FcRH5/IRTA2 Antibody (AF2087) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

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 FCRL5/FcRH5/IRTA2 AF2087
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Verified Customer
Human 11/18/2018
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Summary

Sample Testedhuman tonsil
SpeciesHuman
LotKOM0108111

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Verified Customer
11/18/2018
Application:
Species: Human

Bioinformatics

Gene Symbol FCRL5
Uniprot