NTB-A/SLAMF6/CD352 Antibody (292811) [Unconjugated]

Images

 
Human peripheral blood lymphocytes were stained with Mouse Anti-Human NTB‑A/SLAMF6 Monoclonal Antibody (Catalog # MAB19081, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
292811
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NTB-A/SLAMF6/CD352 Antibody (292811) [Unconjugated] Summary

Additional Information
Clone 292811 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NTB‑A/SLAMF6
Leu28-Lys225
Accession # Q96DU3
Specificity
Detects human NTB‑A/SLAMF6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) BLAME or rhCRACC is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLAMF6
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
MAB19081 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NTB-A/SLAMF6/CD352 Antibody (292811) [Unconjugated]

  • Activating NK receptor
  • CD352 antigen
  • CD352
  • KALIFLJ50657
  • Ly108
  • NK-T-B-antigen
  • NTBA receptor
  • NTBA
  • NTB-A
  • NTB-AMGC104953
  • NTBAT- and B-cell antigen
  • SF2000
  • SLAM family member 6
  • SLAMF6

Background

NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%‑34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5‑7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11).

  1. Veillette, A. (2006) Immunol. Rev. 214:22.
  2. Bottino, C. et al. (2001) J. Exp. Med. 194:235.
  3. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
  4. Munitz, A. et al. (2005) J. Immunol. 174:110.
  5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
  6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
  7. Cao, E. et al. (2006) Immunity 25:559.
  8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
  9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
  10. Howie, D. et al. (2005) J. Immunol. 174:5931.
  11. Kumar, K.R. et al. (2006) Science 312:1665.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NTB-A/SLAMF6/CD352 Antibody (MAB19081)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Neutralization.


Filter By Application
Flow Cytometry
(5)
Neutralization
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB19081 Applications Species
S Joas, EH Parrish, CW Gnanadurai, E Lump, CM Stürzel, NF Parrish, GH Learn, U Sauermann, B Neumann, KM Rensing, D Fuchs, JM Billingsle, SE Bosinger, G Silvestri, C Apetrei, N Huot, T Garcia-Tel, M Müller-Tru, D Hotter, D Sauter, C Stahl-Henn, BH Hahn, F Kirchhoff Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity Nat Commun, 2018;9(1):1371. 2018 [PMID: 29636452] (Flow Cytometry, Human) Flow Cytometry Human
Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruses, 0;33(2):93-100. 0 [PMID: 27296670] (Neutralization, Human) Neutralization Human
Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2011;134(2):167-73. 2011 [PMID: 20933012] (Flow Cytometry, Human) Flow Cytometry Human
Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P New B-cell CD molecules. Immunol. Lett., 2011;134(2):104-12. 2011 [PMID: 20933010] (Flow Cytometry, Human) Flow Cytometry Human
Hislop AD, Palendira U, Leese AM Impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets. Blood, 2010;116(17):3249-57. 2010 [PMID: 20644117] (Flow Cytometry, Human) Flow Cytometry Human
Banerjee P, Feuer G, Barker E Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells. J. Virol., 2007;81(18):9707-17. 2007 [PMID: 17609265] (Flow Cytometry, Human) Flow Cytometry Human

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB19081U
Alexa Fluor 405 FAB19081V
Alexa Fluor 488 FAB19081G
Alexa Fluor 594 FAB19081T
Alexa Fluor 647 FAB19081R
Alexa Fluor 700 FAB19081N
Alexa Fluor 750 FAB19081S
Allophycocyanin FAB19081A
Allophycocyanin FAB19081A
Fluorescein FAB19081F
Fluorescein FAB19081F
Phycoerythrin FAB19081P
Phycoerythrin FAB19081P

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Bioinformatics

Gene Symbol SLAMF6
Uniprot