Fc gamma RIIIB/CD16b Antibody (245514) [Unconjugated]

Product Discontinued

Fc gamma RIIIB/CD16b Antibody (245514) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIB/CD16b
  Thr20-Gln208
  Accession # O75015
• Specificity: Detects human Fc gamma RIIIB/CD16b in direct ELISAs and Western blots. In direct ELISAs, 50% cross-reactivity with human Fc gamma RIIIA is observed. In Western blots, no cross-reactivity with recombinant human Fc gamma RIIA or recombinant mouse Fc gamma RIII is observed. In flow cytometry of whole blood, recognition of Fc gamma RIIIB on the granulocyte population, but not Fc gamma RIIIA on NK cells, is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR3B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIIIB/CD16b Antibody (245514) [Unconjugated]

• CD16
• CD16b antigen
• CD16b
• Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
• Fc gamma RIIIB
• FCG3
• Fc-gamma receptor IIIb (CD 16)
• Fc-gamma RIII
• Fc-gamma RIIIb
• Fc-gamma RIII-beta
• FCGR3
• FCGR3B
• FcgRIIIB
• FcR-10
• fcRIII
• FCRIIIB
• IGFR3
• IgG Fc receptor III-1
• low affinity immunoglobulin gamma Fc region receptor III-B

Background

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1‑3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells, and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RIIIB/CD16b Antibody (MAB1597)(2)

Publications using MAB1597

• S Ceeraz, SK Eszterhas, PA Sergent, DA Armstrong, A Ashare, T Broughton, L Wang, D Pechenick, CM Burns, RJ Noelle, MP Vincenti, RA Fava VISTA deficiency attenuates antibody-induced arthritis and alters macrophage gene expression in response to simulated immune complexes Arthritis Res. Ther., 2017-12-08;19(1):270. 2017-12-08 [PMID: 29216931] (Flow Cytometry, Mouse)
• Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8. J. Clin. Invest., 2010-11-08;120(12):4236-50. 2010-11-08 [PMID: 21060150] (ELISA Development, Flow Cytometry, ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: FCGR3B
• Uniprot:
  • O75015()