Fc gamma RIIIA/CD16a Antibody (CB16) - Azide and BSA Free

Images

 
There are currently no images for Fc gamma RIIIA/CD16a Antibody (NBP2-47951).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
CB16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Fc gamma RIIIA/CD16a Antibody (CB16) - Azide and BSA Free Summary

Immunogen
Human CD16
Localization
Cell Surface
Specificity
It recognizes CD16 (Fcgamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fcgamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • CyTOF-ready
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 1q23 Molecular weight of antigen: 50-80kDa

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Fc gamma RIIIA/CD16a Antibody (CB16) - Azide and BSA Free

  • CD16a antigen
  • CD16a
  • CD16FCRIIIA
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc gamma receptor III-A
  • Fc gamma RIIIA
  • FCG3
  • Fc-gamma receptor III-2 (CD 16)
  • Fc-gamma receptor IIIb (CD16)
  • Fc-gamma RIII
  • Fc-gamma RIIIa
  • Fc-gamma RIII-alpha
  • FCGR3
  • FCGR3A
  • FCGRIII
  • FcgRIIIA
  • FcR-10
  • FCRIII
  • FcRIIIa
  • IGFR3FcgammaRIIIA
  • immunoglobulin G Fc receptor III
  • low affinity III, receptor for (CD16)
  • low affinity immunoglobulin gamma Fc region receptor III-A
  • neutrophil-specific antigen NA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15184
Species: Hu, Rt, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE, Flow-CS
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-89474
Species: Hu, Mu, Rt, Bv
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
AF1330
Species: Hu
Applications: WB, Flow, Block, CyTOF-ready, ICC
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, In vitro, CyTOF-ready
NBP2-14905
Species: Hu
Applications: WB, Flow, ICC/IF
NBP2-04598
Species: Hu
AF1730
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF

Publications for Fc gamma RIIIA/CD16a Antibody (NBP2-47951) (0)

There are no publications for Fc gamma RIIIA/CD16a Antibody (NBP2-47951).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Fc gamma RIIIA/CD16a Antibody (NBP2-47951) (0)

There are no reviews for Fc gamma RIIIA/CD16a Antibody (NBP2-47951). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fc gamma RIIIA/CD16a Antibody (NBP2-47951). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (http://www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
    • The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Fc gamma RIIIA/CD16a Antibody (NBP2-47951)

Discover related pathways, diseases and genes to Fc gamma RIIIA/CD16a Antibody (NBP2-47951). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fc gamma RIIIA/CD16a Antibody (NBP2-47951)

Discover more about diseases related to Fc gamma RIIIA/CD16a Antibody (NBP2-47951).
 

Pathways for Fc gamma RIIIA/CD16a Antibody (NBP2-47951)

View related products by pathway.

PTMs for Fc gamma RIIIA/CD16a Antibody (NBP2-47951)

Learn more about PTMs related to Fc gamma RIIIA/CD16a Antibody (NBP2-47951).
 

Research Areas for Fc gamma RIIIA/CD16a Antibody (NBP2-47951)

Find related products by research area.

Blogs on Fc gamma RIIIA/CD16a

There are no specific blogs for Fc gamma RIIIA/CD16a, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Fc gamma RIIIA/CD16a Antibody (CB16) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol FCGR3A