Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

Western Blot: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunofluorescent analysis. Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:1000. Blue: Fluoroshield with DAPI .

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free [NBP3-25404] - CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. CD32B stained by CD32B antibody [HL1948] (NBP3-25404) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Rt, RM
• Applications: WB, ICC/IF, IHC
• Clone: HL1948
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Fc gamma RIIB/CD32b. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCGR2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Fc gamma RIIB/CD32b Antibody (HL1948) - Azide and BSA Free

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• fcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B