Fc gamma RIIB/CD32b Antibody (110) - Azide and BSA Free

Western Blot: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Lane A: Daudi Whole Cell Lysate Lane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Staining of human lymphonode showing membrane staining of B cells and some other mononuclear cell (1:10000).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 110
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fc gamma RIIB/CD32b Antibody (110) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Fc gamma RIIB/CD32b extracellular domain (Accession#: Ala 46-Pro 217; NP_001002274.1).
• Specificity: Has cross-reactivity in ELISA with:
  
  Human CD64
  Human CD16b
  No cross-reactivity in ELISA with:
  
  Human CD32a
  Human CD16a
  Mouse FCGR2B
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCGR2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2500-1:15000
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Fc gamma RIIB/CD32b Antibody (110) - Azide and BSA Free

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• fcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RIIB/CD32b Antibody (NBP2-89364)(1)

Publication using NBP2-89364

• David A. Knorr, Lucas Blanchard, Rom S. Leidner, Shawn M. Jensen, Ryan Meng, Andrew Jones, Carmen Ballesteros-Merino, Richard B. Bell, Maria Baez, Alessandra Marino, David Sprott, Carlo B. Bifulco, Brian Piening, Rony Dahan, Juan C. Osorio, Bernard A. Fox, Jeffrey V. Ravetch Fc gamma RIIB Is an Immune Checkpoint Limiting the Activity of Treg-Targeting Antibodies in the Tumor Microenvironment Cancer Immunology Research 2024-03-04 [PMID: 38147316]

Bioinformatics

• Gene Symbol: FCGR2B