Fc gamma RII/CD32 Antibody (AT10) [Biotin]

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [Biotin] [NB100-63713] - Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.

• Reactivity: Hu
• Applications: Flow
• Clone: AT10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: 0.1 mg/ml

Fc gamma RII/CD32 Antibody (AT10) [Biotin] Summary

• Immunogen: K562 cell line
• Specificity: NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: This antibody does not work in Immunohistochemistry-Paraffin.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371
• Control:

  Fc gamma RIIB/CD32b Overexpression Lysate

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey, Dog, Porcine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% Bovine Serum Albumin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for Fc gamma RII/CD32 Antibody (AT10) [Biotin]

• CD32 antigen
• CD32B
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RII
• Fc gamma RIIb
• FCG2
• Fc-gamma RII-b
• FcgammaRIIb
• fc-gamma-RIIb
• FCGR2
• FcgRII
• FcgRIIB
• fcRII-b
• FcRII-c
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RII/CD32 Antibody (NB100-63713)(1)

Publication using NB100-63713

• Sun D, Raisley B, Langer M et al. Anti-peptidoglycan antibodies and fc gamma receptors are the key mediators of inflammation in gram-positive sepsis J Immunol 2012-09-01 [PMID: 22815288] (FLOW, Human)
  
  Details:
  This citation used the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: FCGR2B