Fc gamma RII/CD32 Antibody (AT10)

Images

 
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the Biotin conjugate of NB100-65338. Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.

Product Details

Summary
Reactivity Hu, Po, Ca, PmSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
AT10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Fc gamma RII/CD32 Antibody (AT10) Summary

Immunogen
K562 cell line
Specificity
NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunoprecipitation 20 ug/ml
Application Notes
This antibody does not work in Immunohistochemistry-Paraffin. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections.
Control
Fc gamma RIIB/CD32b Lysate (NBL1-10656)
Reviewed Applications
Read 1 Review rated 4
using
NB100-65338 in the following applications:

Publications
Read Publications using
NB100-65338 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Fc gamma RII/CD32 Antibody (AT10)

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • fcRII-b
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, IHC, IHC-Fr, IP

Publications for Fc gamma RII/CD32 Antibody (NB100-65338)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, Flow.


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FLOW
(1)
Flow
(1)
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Human
(2)
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Showing Publications 1 - 2 of 2.
Publications using NB100-65338 Applications Species
Sun D, Raisley B, Langer M et al. Anti-peptidoglycan antibodies and fc gamma receptors are the key mediators of inflammation in gram-positive sepsis J Immunol (FLOW, Human)

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB100-63713).
FLOW Human
Sun D, Raisley B, Langer M et al. Anti-peptidoglycan antibodies and fc gamma receptors are the key mediators of inflammation in gram-positive sepsis J Immunol 2012 Sep 1 [PMID: 22815288] (Flow, Human)

Details:
This citation used the Biotin version of this antibody.
Flow Human

Review for Fc gamma RII/CD32 Antibody (NB100-65338) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-65338:
Filter by Applications
IP
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IP Human 11/16/2011
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Summary

ApplicationImmunoprecipitation
Sample TestedHuman
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Product General Protocols

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FAQs for Fc gamma RII/CD32 Antibody (NB100-65338) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65338AF405
Alexa Fluor 488 Labeled NB100-65338AF488
Alexa Fluor 647 Labeled NB100-65338AF647
Alexa Fluor 700 Labeled NB100-65338AF700
Allophycocyanin Labeled NB100-65338APC
Biotin Labeled NB100-63713
DyLight 350 Labeled NB100-65338UV
DyLight 405 Labeled NB100-65338V
DyLight 488 Labeled NB100-65338G
DyLight 680 Labeled NB100-65338FR
DyLight 755 Labeled NB100-65338IR
FITC Labeled NB100-63714
PE Labeled NB100-65338PE
PerCP Labeled NB100-65338PCP

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Bioinformatics Tool for Fc gamma RII/CD32 Antibody (NB100-65338)

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Recent Reviews

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4
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0
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1
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Anonymous
11/16/2011
Application: IP
Species: Human

Bioinformatics

Gene Symbol FCGR2B