FBXO8 Antibody

Images

 
Western Blot: FBXO8 Antibody [NBP2-16453] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: FBXO8 Antibody [NBP2-16453] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: FBXO8 Antibody [NBP2-16453] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FBXO8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FBXO8. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXO8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (91%), Xenopus tropicalis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FBXO8 Antibody

  • F-box only protein 8
  • F-box protein 8
  • F-box/SEC7 protein FBS
  • FBSFBX8F-box protein Fbx8

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FBXO8
Uniprot