FBXO11 Antibody

Images

 
Genetic Strategies: Knockout Validated: FBXO11 Antibody [NB100-59826] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FBXO11 knockout (KO) HeLa cell line. PVDF ...read more
Independent Antibodies: Western Blot: FBXO11 Antibody [NB100-59826] - Detection of Human FBXO11 on HeLa whole cell lysate using NB100-59826. FBXO11 was also immunoprecipitated by rabbit anti-FBXO11 antibody ...read more
Genetic Strategies: Western Blot: FBXO11 Antibody [NB100-59826] - (A) Breast cancer cell line MDA-MB231-LM2 with/without FBXO11 knockdown. (B) 4T1 xenograft tumors with/without FBXO11 knockdown. Image from ...read more
Western Blot: FBXO11 Antibody [NB100-59826] - Analysis of FBXO11 in Raji whole cell lysate (40 ug/lane) using anti-FBXO11 antibody (Cat. # NB100-59826). Image from verified customer review.
Simple Western: FBXO11 Antibody [NB100-59826] - Simple Western lane view shows a specific band for FBXO11 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

FBXO11 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 877 and 927 of human F-box only protein 11 using the numbering given in entry NP_079409.3 (GeneID 80204).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXO11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Knockout Validated
  • Simple Western 1:40
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:34505148). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. FBXO11antibody validated for WB from verified customer reviews.
Control
FBXO11 Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-59826 in the following applications:

Publications
Read Publications using
NB100-59826 in the following applications:

  • 1 publication
  • SW
    1 publication
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FBXO11 Antibody

  • F-box only protein 11
  • F-box protein 11
  • FBX11MGC44383
  • FLJ12673
  • PRMT9VIT1protein arginine N-methyltransferase 9
  • ubiquitin protein ligase E3 component n-recognin 6
  • UBR6
  • VIT-1
  • Vitiligo-associated protein 1
  • vitiligo-associated protein VIT-1

Background

FBXO11 is a member of the F-box protein family and component of the Skp1-Cullin1-F-box (SCF) ubiquitin ligase complex. FBXO11 has been shown to interact with p53 and suppress its activity. Suppression of p53 activity is achieved via Nedd8 conjugation to p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rb, Rt, Sh
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Species: Fi, Ha, Hu, Mu, Pm, Rb, Rt, Re, Sh
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, PAGE, Simple Western, WB
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Species: Hu, Pm, Mu
Applications: IHC, IHC-P, WB
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Species: Hu
Applications: BA
NB300-130
Species: Bv, Ce, Ch, Dr, Eq, Hu, Pm, Mu, Pl, Po, Rt, Ze
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, WB
H00007444-M01
Species: Hu
Applications: ELISA, ICC/IF, KD, S-ELISA, WB
NB600-101
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
NB100-59826
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IP, KO

Publications for FBXO11 Antibody (NB100-59826)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, SW, WB.


Filter By Application
ICC/IF
(1)
SW
(1)
WB
(4)
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Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-59826 Applications Species
Gregor A, Meerbrei T, Gerstner T et al. De novo missense variants in FBXO11 alter its protein expression and subcellular localization Human molecular genetics Sep 9 2021 [PMID: 34505148] (ICC/IF, Human) ICC/IF Human
White MR, Mitrea DM, Zhang P et al. Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2Fs Mol. Cell May 17 2019 [PMID: 31130363]
Horn M, Geisen C, Cermak L et al. DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Dev. Cell 3/4/2014 [PMID: 24613396] (WB, Human) WB Human
Rossi M, Duan S, Jeong YT et al. Regulation of the CRL4(Cdt2) Ubiquitin Ligase and Cell-Cycle Exit by the SCF(Fbxo11) Ubiquitin Ligase Mol Cell 2013 Mar 28 [PMID: 23478441] (WB, Human) WB Human
Duan S, Cermak L, Pagan JK et al. FBXO11 targets BCL6 for degradation and is inactivated in diffuse large B-cell lymphomas. Nature. 2011 Nov 23. [PMID: 22113614]
Barra J, Gaidosh G, Blumenthal E et al. Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1 Sci. Adv. Jul 1 2020 (WB, Human) WB Human
Schieber M, Marinaccio C, Bolanos LC et al. FBXO11 is a candidate tumor suppressor in the leukemic transformation of myelodysplastic syndrome Blood Cancer J Oct 6 2020 [PMID: 33024076] (WB, Human) WB Human
Barra, J, Gaidosh, G S Et al. Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Sci Adv Jul 1 2020 [PMID: 32923585] (SW, Mouse) SW Mouse

Reviews for FBXO11 Antibody (NB100-59826) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Human and Mouse.

Reviews using NB100-59826:
Filter by Applications
Western Blot
(4)
All Applications
Filter by Species
Human
(3)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FBXO11 NB100-59826
Enlarge
5
reviewed by:
Minhong Shen
Western Blot Human and Mouse 12/11/2018
View

Summary

ApplicationWestern Blot
Sample Tested4T1 cells, MDA-MB-231, T-47D, ZR-71 whole cell extracts,4T1 mouse breast cancer cell line,Breast tumor
SpeciesHuman and Mouse
LotCannot find
Western Blot FBXO11 NB100-59826
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 02/10/2017
View

Summary

ApplicationWestern Blot
Sample TestedB cell leukemia,B cell lymphoma,Jurkat human acute T cell leukemia cell line
SpeciesHuman
  4
reviewed by:
cynthia morimoto
Western Blot Human 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 23478441
SpeciesHuman
Western Blot FBXO11 NB100-59826
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 10/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedRaji whole cell lysate
SpeciesHuman
LotA1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FBXO11 Antibody (NB100-59826) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Minhong Shen
12/11/2018
Application: Western Blot
Species: Human and Mouse

Verified Customer
02/10/2017
Application: Western Blot
Species: Human

 
cynthia morimoto
12/19/2014
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol FBXO11
Entrez
Uniprot