FBXO11 Antibody

Western Blot: FBXO11 Antibody [NB100-59826] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FBXO11 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human FBXO11 Polyclonal Antibody (Catalog # NB100-59826) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FBXO11 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: FBXO11 Antibody [NB100-59826] - Detection of Human FBXO11 on HeLa whole cell lysate using NB100-59826. FBXO11 was also immunoprecipitated by rabbit anti-FBXO11 antibody NB100-59825.

Western Blot: FBXO11 Antibody [NB100-59826] - (A) Breast cancer cell line MDA-MB231-LM2 with/without FBXO11 knockdown. (B) 4T1 xenograft tumors with/without FBXO11 knockdown. Image from verified customer review.

Western Blot: FBXO11 Antibody [NB100-59826] - Analysis of FBXO11 in Raji whole cell lysate (40 ug/lane) using anti-FBXO11 antibody (Cat. # NB100-59826). Image from verified customer review.

Simple Western: FBXO11 Antibody [NB100-59826] - Simple Western lane view shows a specific band for FBXO11 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FBXO11 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 877 and 927 of human F-box only protein 11 using the numbering given in entry NP_079409.3 (GeneID 80204).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXO11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Knockout Validated
  • Simple Western 1:40
  • Western Blot 1:2000-1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:34505148). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:40, apparent MW was 140 kDa. FBXO11 antibody validated for WB from verified customer reviews.
• Control:

  FBXO11 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXO11 Antibody

• F-box only protein 11
• F-box protein 11
• FBX11MGC44383
• FLJ12673
• PRMT9VIT1protein arginine N-methyltransferase 9
• ubiquitin protein ligase E3 component n-recognin 6
• UBR6
• VIT-1
• Vitiligo-associated protein 1
• vitiligo-associated protein VIT-1

Background

FBXO11 is a member of the F-box protein family and component of the Skp1-Cullin1-F-box (SCF) ubiquitin ligase complex. FBXO11 has been shown to interact with p53 and suppress its activity. Suppression of p53 activity is achieved via Nedd8 conjugation to p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXO11 Antibody (NB100-59826)(12)

Publications using NB100-59826

• Zhuang X, Woods J, Ji Y et al. Functional genomics identifies extension of complex N-glycans as a mechanism to evade lysis by natural killer cells bioRxiv 2023-04-05 (WB, Mouse)
• Jeon Y, Chow SH, Stuart I et al. FBXO11 governs macrophage cell death and inflammation in response to bacterial toxins Life science alliance 2023-06-01 [PMID: 36977592] (WB, Human)
• Duan S, Cermak L, Pagan JK et al. FBXO11 targets BCL6 for degradation and is inactivated in diffuse large B-cell lymphomas. Nature 2012-01-01 [PMID: 22113614]
• Simon C, Brunke I, Stielow B et al. SAMD1 suppresses epithelial-mesenchymal transition (EMT) pathways in pancreatic ductal adenocarcinoma bioRxiv 2023-08-15 (WB, Human)
• Zhuang, X;Woods, J;Ji, Y;Scheich, S;Mo, F;Rajagopalan, S;Coulibaly, ZA;Voss, M;Urlaub, H;Staudt, LM;Pan, KT;Long, EO; Functional genomics identifies N-acetyllactosamine extension of complex N-glycans as a mechanism to evade lysis by natural killer cells Cell reports 2024-04-14 [PMID: 38619967]
• Chan KL, Gomez J, Cardinez C, Kumari N et Al. Inhibition of the CtBP complex and FBXO11 enhances MHC class II expression and anti-cancer immune responses Cancer Cell 2022-09-30 [PMID: 36179686]
• Gregor A, Meerbrei T, Gerstner T et al. De novo missense variants in FBXO11 alter its protein expression and subcellular localization Human molecular genetics 2021-09-09 [PMID: 34505148] (ICC/IF, Human)
• Schieber M, Marinaccio C, Bolanos LC et al. FBXO11 is a candidate tumor suppressor in the leukemic transformation of myelodysplastic syndrome Blood Cancer J 2020-10-06 [PMID: 33024076] (WB, Human)
• Barra, J, Gaidosh, G S Et al. Integrator restrains paraspeckles assembly by promoting isoform switching of the lncRNA NEAT1. Sci Adv 2020-07-01 [PMID: 32923585] (WB, Mouse)
• White MR, Mitrea DM, Zhang P et al. Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2Fs Mol. Cell 2019-05-17 [PMID: 31130363]
• Horn M, Geisen C, Cermak L et al. DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Dev. Cell 2014-03-04 [PMID: 24613396] (WB, Human)
• Rossi M, Duan S, Jeong YT et al. Regulation of the CRL4(Cdt2) Ubiquitin Ligase and Cell-Cycle Exit by the SCF(Fbxo11) Ubiquitin Ligase Mol Cell 2013-03-28 [PMID: 23478441] (WB, Human)

Reviews for FBXO11 Antibody (NB100-59826) (4)

Average Rating: 4.5

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Human and Mouse.

reviewed by: Verified Customer WB Human and Mouse 12/11/2018

Summary

• Application: Western Blot
• Sample Tested: 4T1 cells, MDA-MB-231, T-47D, ZR-71 whole cell extracts,4T1 mouse breast cancer cell line,Breast tumor
• Species: Human and Mouse
• Lot: Cannot find

reviewed by: JAEWOONG LEE WB Human 02/10/2017

Summary

• Application: Western Blot
• Sample Tested: B cell leukemia,B cell lymphoma,Jurkat human acute T cell leukemia cell line
• Species: Human

reviewed by: Verified Customer WB Human 12/19/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID: 23478441
• Species: Human

reviewed by: Xiaoqing Lu WB Human 10/02/2014

Summary

• Application: Western Blot
• Sample Tested: Raji whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: FBXO11
• Entrez:
  • 80204(Human)
• Uniprot:
  • A1L491(Human)