FBXO11 Antibody

Images

 
Genetic Strategies: Knockout Validated: FBXO11 Antibody [NB100-59826] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FBXO11 knockout (KO) HeLa cell line. PVDF ...read more
Independent Antibodies: Western Blot: FBXO11 Antibody [NB100-59826] - Detection of Human FBXO11 on HeLa whole cell lysate using NB100-59826. FBXO11 was also immunoprecipitated by rabbit anti-FBXO11 antibody ...read more
Western Blot: FBXO11 Antibody [NB100-59826] - Analysis of FBXO11 in Raji whole cell lysate (40 ug/lane) using anti-FBXO11 antibody (Cat. # NB100-59826). Image from verified customer review.
Simple Western: FBXO11 Antibody [NB100-59826] - Simple Western lane view shows a specific band for FBXO11 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

FBXO11 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 877 and 927 of human F-box only protein 11 using the numbering given in entry NP_079409.3 (GeneID 80204).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXO11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Simple Western 1:40
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockout Validated
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Positive Control
FBXO11 Lysate (NBL1-10620)
FBXO11 Lysate (NBP2-65327)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-59826 in the following applications:

Publications
Read Publications using
NB100-59826 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FBXO11 Antibody

  • F-box only protein 11
  • F-box protein 11
  • FBX11MGC44383
  • FLJ12673
  • PRMT9VIT1protein arginine N-methyltransferase 9
  • ubiquitin protein ligase E3 component n-recognin 6
  • UBR6
  • VIT-1
  • Vitiligo-associated protein 1
  • vitiligo-associated protein VIT-1

Background

FBXO11 is a member of the F-box protein family and component of the Skp1-Cullin1-F-box (SCF) ubiquitin ligase complex. FBXO11 has been shown to interact with p53 and suppress its activity. Suppression of p53 activity is achieved via Nedd8 conjugation to p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, Simple Western, IP, KO

Publications for FBXO11 Antibody (NB100-59826)(3)

Reviews for FBXO11 Antibody (NB100-59826) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-59826:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot FBXO11 NB100-59826
Enlarge
reviewed by:
JAEWOONG LEE
WB Human 02/10/2017
View

Summary

ApplicationWestern Blot
Sample TestedB cell lymphoma,B cell leukemia,Jurkat human acute T cell leukemia cell line
SpeciesHuman
 
reviewed by:
Anonymous
WB Human 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 23478441
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 23478441

Details

Detection NotesSee PMID: 23478441

Comments

CommentsPublished in PMID: 23478441.Independent review from antibodyresource.com
Western Blot FBXO11 NB100-59826
Enlarge
reviewed by:
Xiaoqing Lu
WB Human 10/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedRaji whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% non fat milk in PBS with 0.1% Tween 20, 1 hour at 22C

Primary Anitbody

Dilution Ratio1:500, incubated for 16 hours at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP
Secondary Manufacturer Cat#sc-2030
Secondary Concentration1:5000 dilution from 400ug/ml stock

Details

Detection NotesECL+, exposure time 1 minute.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FBXO11 Antibody (NB100-59826) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FBXO11 Antibody (NB100-59826)

Discover related pathways, diseases and genes to FBXO11 Antibody (NB100-59826). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on FBXO11

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

JAEWOONG LEE
02/10/2017
Application: WB
Species: Human

 
Anonymous
12/19/2014
Application: WB
Species: Human

Xiaoqing Lu
10/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol FBXO11
Entrez
Uniprot