VRK2 Antibody (3B10)

Western Blot: VRK2 Antibody (3B10) [H00007444-M01] - VRK2 monoclonal antibody (M01), clone 3B10 Analysis of VRK2 expression in K-562.

Immunocytochemistry/Immunofluorescence: VRK2 Antibody (3B10) [H00007444-M01] - Analysis of monoclonal antibody to VRK2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: VRK2 Antibody (3B10) [H00007444-M01] - Analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi ( Cat # H00007444-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody (M01) clone 3B10 (Cat # H00007444-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: VRK2 Antibody (3B10) [H00007444-M01] - VRK2 monoclonal antibody (M01), clone 3B10. Analysis of VRK2 expression in U-2 OS.

Western Blot: VRK2 Antibody (3B10) [H00007444-M01] - Analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody (M01), clone 3B10.Lane 1: VRK2 transfected lysate(58.1 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: VRK2 Antibody (3B10) [H00007444-M01] - Detection limit for recombinant GST tagged VRK2 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 3B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VRK2 Antibody (3B10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQTAKTNLLDELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKAHNRLI
• Specificity: VRK2 - vaccinia related kinase 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VRK2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VRK2 Antibody (3B10)

• EC 2.7.11.1
• serine/threonine-protein kinase VRK2
• vaccinia related kinase 2
• vaccinia virus B1R-related kinase 2
• Vaccinia-related kinase 2
• vaccinia-related kinase-2

Background

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. While several transcript variants may exist for this gene, the full-length nature of only one has been biologically validated to date.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VRK2
• Entrez:
  • 7444(Human)
• OMIM:
  • 602169(Human)
• Uniprot:
  • AAH27854(Human)