Nogo Antibody

Images

 
Western Blot: Nogo Antibody [NB100-56681] - Analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - Analysis of human testis using Nogo antibody at 1:100.
Simple Western: Nogo Antibody [NB100-56681] - Lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, ShSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Nogo Antibody Summary

Immunogen
A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].
Specificity
This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTN4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:1000
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively). Use in ICC/IF reported in scientific literature (PMID 27132996). Use in Immunohistochemistry reported in scientific literature (PMID: 30208932). Use in ELISA reported in scientific literature (PMID: 17413036).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Rat Brain Whole Tissue Lysate (Adult Whole Normal)
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Nogo Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-56681 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 24223763).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Nogo Antibody

  • ASY
  • foocen
  • Human NogoA
  • KIAA0886
  • My043 protein
  • Nbla00271
  • Nbla10545
  • neurite growth inhibitor 220
  • Neurite outgrowth inhibitor
  • Neuroendocrine-specific protein C homolog
  • Neuroendocrine-specific protein
  • NI220/250
  • Nogo protein
  • NOGO-A
  • Nogo-B
  • NOGOC
  • Nogo-C
  • NOGORTN4-B1
  • NSP
  • NSP-CL
  • reticulon 4
  • reticulon 5
  • reticulon-4
  • reticulon-5
  • RTN4-A
  • RTN4-B2
  • RTN4-C
  • RTN-x

Background

Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Nogo Antibody (NB100-56681)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Rabbit, Sheep.

We have publications tested in 5 applications: ELISA, ICC/IF, IHC, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(4)
IHC
(2)
IHC-P
(1)
WB
(7)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Rat
(4)
Rabbit
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-56681 Applications Species
Zhang S, Yu M, Guo F et al. Rosiglitazone alleviates alpha-naphthylisothiocyanate-induced intrahepatic cholestasis in mice the role of circulating 15d-PGJ2 and Nogo Br. J. Pharmacol. Oct 28 2019 [PMID: 31658492]
Chino, H;Hatta, T;Natsume, T;Mizushima, N; Intrinsically Disordered Protein TEX264 Mediates ER-phagy Mol. Cell Apr 11 2019 [PMID: 31006538] (WB, Human) WB Human
Farias GG, Freal A, Tortosa E et al. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity Neuron Feb 13 2019 [PMID: 30772082] (ICC/IF, Rat) ICC/IF Rat
Gao P, Wang X, Jin Y et al. Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells. Breast Cancer Res. Sep 12 2018 [PMID: 30208932] (IHC) IHC
Kawaguchi N, Tashiro K, Taniguchi K et al. Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells Biochim. Biophys. Acta 2018 Apr 20 [PMID: 29684585] (WB, IHC, Human) WB, IHC Human
Jin Y, Hu W, Liu T et al. Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel. Cancer Lett. 2018 Apr 10 [PMID: 29373839] (Human) Human
Teng RJ, Rana U, Afolayan AJ et al. Nogo-B Receptor Modulates Angiogenesis Response of Pulmonary Artery Endothelial Cells Through eNOS Coupling. Am. J. Respir. Cell Mol. Biol. 2014 Feb 25 [PMID: 24568601] (IHC-P, WB, Sheep)

Details:
IHC-P (lung), Fig 1A-D; WB [lung, pulmonary artery endothelial cells (PAEC)], Fig 1E-F. For IHC-P, the Nogo pAb was used at 1:4000. Two bands were observed in WB for Nogo in both lung and PAEC, similar to previous reports in the literature. These bands we
IHC-P, WB Sheep
Jozsef L, Tashiro K, Kuo A et al. Reticulon 4 is necessary for ER tubulation, STIM1-Orai1 coupling and store-operated calcium entry. J. Biol. Chem. 2014 Mar 11 [PMID: 24558039] (ICC/IF, WB, Mouse)

Details:
Mouse embryo fibroblasts (MEFs) including wildytpe (WT), knockout (kO) and RTN4b stably transfected: WB (Figs 1A, 1C, 1D; 7A, 8A, 8D), IF (Figs 1B, 12A)Note: The specificity of the pAb was validated in RTN-kO versus wildtype MEFs by WB, and in RTN4b stabl
ICC/IF, WB Mouse
Chan RT, Russell RA, Marcal H et al. BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration. Biomacromolecules 2014 Jan 13 [PMID: 24299034] (WB, Rat) WB Rat
Wang B, Zhao B, North P et al. Expression of NgBR Is Highly Associated with Estrogen Receptor Alpha and Survivin in Breast Cancer. PLoS One 2013 Nov 4 [PMID: 24223763] (Rabbit) Rabbit
Show All 14 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nogo Antibody (NB100-56681) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nogo Antibody (NB100-56681)

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Blogs on Nogo.

Nogo: A Promising Target for New Gene Therapies
Nogo is a neurite outgrowth inhibitor protein that plays an important role during central nervous system (CNS) development as well as in endoplasmic reticulum signaling regulation. Studies using Nogo antibodies have revealed Nogo proteins regulate...  Read full blog post.

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Bioinformatics

Gene Symbol RTN4
Entrez
Uniprot