Nogo Antibody

Images

 
Western Blot: Nogo Antibody [NB100-56681] - analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - analysis of human testis using Nogo antibody at 1:100.
Simple Western: Nogo Antibody [NB100-56681] - Simple Western lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Nogo Antibody Summary

Immunogen
A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].
Specificity
This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTN4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27132996).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Brain Lysate (NB820-59705)
Brain Lysate (NB820-59657)
Brain Lysate (NB820-59177)
Publications
Read Publications using
NB100-56681 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Nogo Antibody

  • ASY
  • foocen
  • Human NogoA
  • KIAA0886
  • My043 protein
  • Nbla00271
  • Nbla10545
  • neurite growth inhibitor 220
  • Neurite outgrowth inhibitor
  • Neuroendocrine-specific protein C homolog
  • Neuroendocrine-specific protein
  • NI220/250
  • Nogo protein
  • NOGO-A
  • Nogo-B
  • NOGOC
  • Nogo-C
  • NOGORTN4-B1
  • NSP
  • NSP-CL
  • reticulon 4
  • reticulon 5
  • reticulon-4
  • reticulon-5
  • RTN4-A
  • RTN4-B2
  • RTN4-C
  • RTN-x

Background

Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Nogo Antibody (NB100-56681)(8)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Rabbit, Sheep.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(3)
IHC-P
(1)
WB
(5)
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Human
(1)
Mouse
(2)
Rat
(3)
Rabbit
(1)
Sheep
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB100-56681 Applications Species
Rodriguez-Feo JA, Gallego-Delgado J, Puerto M et al. Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells. Biochim. Biophys. Acta. Apr 28 2016 [PMID: 27132996] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Teng RJ, Rana U, Afolayan AJ et al. Nogo-B Receptor Modulates Angiogenesis Response of Pulmonary Artery Endothelial Cells Through eNOS Coupling. Am. J. Respir. Cell Mol. Biol. 2014 Feb 25 [PMID: 24568601] (IHC-P, WB, Sheep)

Details:
IHC-P (lung), Fig 1A-D; WB [lung, pulmonary artery endothelial cells (PAEC)], Fig 1E-F. For IHC-P, the Nogo pAb was used at 1:4000. Two bands were observed in WB for Nogo in both lung and PAEC, similar to previous reports in the literature. These bands we
IHC-P, WB Sheep
Jozsef L, Tashiro K, Kuo A et al. Reticulon 4 is necessary for ER tubulation, STIM1-Orai1 coupling and store-operated calcium entry. J. Biol. Chem. 2014 Mar 11 [PMID: 24558039] (ICC/IF, WB, Mouse)

Details:
Mouse embryo fibroblasts (MEFs) including wildytpe (WT), knockout (kO) and RTN4b stably transfected: WB (Figs 1A, 1C, 1D; 7A, 8A, 8D), IF (Figs 1B, 12A)Note: The specificity of the pAb was validated in RTN-kO versus wildtype MEFs by WB, and in RTN4b stabl
ICC/IF, WB Mouse
Chan RT, Russell RA, Marcal H et al. BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration. Biomacromolecules 2014 Jan 13 [PMID: 24299034] (WB, Rat) WB Rat
Wang B, Zhao B, North P et al. Expression of NgBR Is Highly Associated with Estrogen Receptor Alpha and Survivin in Breast Cancer. PLoS One 2013 Nov 4 [PMID: 24223763] (Rabbit) Rabbit
Gramolini AO, Kislinger T, Alikhani-Koopaei R et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008 Mar [PMID: 18056057] (WB, Mouse)

Details:
WB, mouse heart tissue: 1. IMG- 5346A (Nogo A/B): Fig. C. 2. IMG-3374 (FHL1): Figs. 3C; 4A, B; 6; 8A, B.
WB Mouse
Rodriguez-Feo JA, Hellings WE, Verhoeven BA et al. Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity. Arterioscler Thromb Vasc Biol. 2007 Jun [PMID: 17413036] (ELISA, Human)

Details:
Nogo-A/B (IMG-5346A). ELISA: Human plasma, Fig 3.
ELISA Human
Batenburg V. Mapping the Neuronal Cytoskeleton with Expansion Microscopy. Thesis. 2017 Oct 15 (ICC/IF, Rat) ICC/IF Rat

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Nogo.

Nogo: A Promising Target for New Gene Therapies
Nogo is a neurite outgrowth inhibitor protein that plays an important role during central nervous system (CNS) development as well as in endoplasmic reticulum signaling regulation. Studies using Nogo antibodies have revealed Nogo proteins regulate...  Read full blog post.

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Bioinformatics

Gene Symbol RTN4
Entrez
Uniprot