Nogo Antibody


Western Blot: Nogo Antibody [NB100-56681] - analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - analysis of human testis using Nogo antibody at 1:100.
Simple Western: Nogo Antibody [NB100-56681] - Simple Western lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-P
1.0 mg/ml

Nogo Antibody Summary

A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].
This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 1:500-1:2000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27132996).
Read Publications using
NB100-56681 in the following applications:

Alternate Names for Nogo Antibody

  • ASY
  • foocen
  • Human NogoA
  • KIAA0886
  • My043 protein
  • Nbla00271
  • Nbla10545
  • neurite growth inhibitor 220
  • Neurite outgrowth inhibitor
  • Neuroendocrine-specific protein C homolog
  • Neuroendocrine-specific protein
  • NI220/250
  • Nogo protein
  • NOGO-A
  • Nogo-B
  • Nogo-C
  • NSP
  • NSP-CL
  • reticulon 4
  • reticulon 5
  • reticulon-4
  • reticulon-5
  • RTN4-A
  • RTN4-B2
  • RTN4-C
  • RTN-x


Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nogo Antibody (NB100-56681)(7)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Rabbit, Sheep.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC-P, WB.

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Showing Publications 1 - 7 of 7.
Publications using NB100-56681 Applications Species
Rodriguez-Feo JA, Gallego-Delgado J, Puerto M et al. Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells. Biochim. Biophys. Acta. Apr 28 2016 12:00AM [PMID:27132996] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Teng RJ, Rana U, Afolayan AJ et al. Nogo-B Receptor Modulates Angiogenesis Response of Pulmonary Artery Endothelial Cells Through eNOS Coupling. Am. J. Respir. Cell Mol. Biol. 2014 Feb 25 [PMID:24568601] (IHC-P, WB, Sheep) IHC-P, WB Sheep
Jozsef L, Tashiro K, Kuo A et al. Reticulon 4 is necessary for ER tubulation, STIM1-Orai1 coupling and store-operated calcium entry. J. Biol. Chem. 2014 Mar 11 [PMID:24558039] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Chan RT, Russell RA, Marcal H et al. BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration. Biomacromolecules 2014 Jan 13 [PMID:24299034] (WB, Rat) WB Rat
Wang B, Zhao B, North P et al. Expression of NgBR Is Highly Associated with Estrogen Receptor Alpha and Survivin in Breast Cancer. PLoS One 2013 Nov 4 [PMID:24223763] (Rabbit) Rabbit
Gramolini AO, Kislinger T, Alikhani-Koopaei R et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008 Mar [PMID:18056057] (WB, Mouse) WB Mouse
Rodriguez-Feo JA, Hellings WE, Verhoeven BA et al. Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity. Arterioscler Thromb Vasc Biol. 2007 Jun [PMID:17413036] (ELISA, Human) ELISA Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nogo Antibody (NB100-56681) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NB100-56681AF488
Alexa Fluor 647 Labeled NB100-56681AF647
Allophycocyanin Labeled NB100-56681APC
Biotin Labeled NB100-56681B
DyLight 350 Labeled NB100-56681UV
DyLight 405 Labeled NB100-56681V
DyLight 405LS Labeled NB100-56681V3
DyLight 488 Labeled NB100-56681G
DyLight 550 Labeled NB100-56681R
DyLight 650 Labeled NB100-56681C
DyLight 680 Labeled NB100-56681FR
DyLight 755 Labeled NB100-56681IR
HRP Labeled NB100-56681H
PE Labeled NB100-56681PE
PerCP Labeled NB100-56681PCP

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Blogs on Nogo.

Nogo: A Promising Target for New Gene Therapies
Nogo is a neurite outgrowth inhibitor protein that plays an important role during central nervous system (CNS) development as well as in endoplasmic reticulum signaling regulation. Studies using Nogo antibodies have revealed Nogo proteins regulate...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol RTN4

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