Western Blot: Nogo Antibody [NB100-56681] - analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - analysis of human testis using Nogo antibody at 1:100.
Simple Western: Nogo Antibody [NB100-56681] - Simple Western lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Western Blot 1:500-1:2000
Simple Western 1:1000
This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27132996).
Read Publications using NB100-56681 in the following applications:
Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.