Nogo Antibody

Western Blot: Nogo Antibody [NB100-56681] - Analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.

Immunocytochemistry/Immunofluorescence: Nogo Antibody [NB100-56681] - Increased lipid droplet biogenesis buffers lack spastin-M1 at the ER. MEF cell lines were starved in HBSS and treated either with inhibitors of DGAT1 and DGAT2 (D1+D2i) or DMSO overnight and stained with antibodies against Nogo antibody. Representative single-plane confocal images. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC7184029/) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - Analysis of human testis using Nogo antibody at 1:100.

Simple Western: Nogo Antibody [NB100-56681] - Lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Rb, Sh
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Nogo Antibody Summary

• Immunogen: A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].
• Specificity: This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RTN4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively). Use in ICC/IF reported in scientific literature (PMID 27132996). Use in Immunohistochemistry reported in scientific literature (PMID: 30208932). Use in ELISA reported in scientific literature (PMID: 17413036).
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 24223763).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Nogo Antibody

• ASY
• foocen
• Human NogoA
• KIAA0886
• My043 protein
• Nbla00271
• Nbla10545
• neurite growth inhibitor 220
• Neurite outgrowth inhibitor
• Neuroendocrine-specific protein C homolog
• Neuroendocrine-specific protein
• NI220/250
• Nogo protein
• NOGO-A
• Nogo-B
• NOGOC
• Nogo-C
• NOGORTN4-B1
• NSP
• NSP-CL
• reticulon 4
• reticulon 5
• reticulon-4
• reticulon-5
• RTN4-A
• RTN4-B2
• RTN4-C
• RTN-x

Background

Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nogo Antibody (NB100-56681)(19)

Publications using NB100-56681

• Wang X, Yang Y, Zhao D et al. Inhibition of high-fat diet-induced obesity via reducing of ER-resident protein Nogo occurs through multiple mechanisms Journal of Biological Chemistry 2022-01-01 [PMID: 34998825] (IHC-P, Mouse)
• Yousefi R, Jevdokimenko K, Kluever V Et Al. Influence of Subcellular Localization and Functional State on Protein Turnover Cells 2021-08-07 [PMID: 34359917]
• Zahavi EE, Hummel JJA, Han Y et al. Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriers Developmental cell 2021-02-22 [PMID: 33571451]
• Batenburg V. Mapping the Neuronal Cytoskeleton with Expansion Microscopy. Methods Cell Biol 2021-01-22 [PMID: 33478685]
• Petrova V, Pearson CS, Ching J et al. Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS Nat Commun 2020-11-05 [PMID: 33154382]
• Tadepalle N, Robers L et al. Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesis. Life Sci Alliance 2020-01-06 [PMID: 32321733] (ICC/IF, Mouse)
• Zhang S, Yu M, Guo F et al. Rosiglitazone alleviates alpha-naphthylisothiocyanate-induced intrahepatic cholestasis in mice the role of circulating 15d-PGJ2 and Nogo Br. J. Pharmacol. 2019-10-28 [PMID: 31658492]
• Chino, H;Hatta, T;Natsume, T;Mizushima, N; Intrinsically Disordered Protein TEX264 Mediates ER-phagy Mol. Cell 2019-04-11 [PMID: 31006538] (WB, Human)
• Farias GG, Freal A, Tortosa E et al. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity Neuron 2019-02-13 [PMID: 30772082] (ICC/IF, Rat)
• Gao P, Wang X, Jin Y et al. Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells. Breast Cancer Res. 2018-09-12 [PMID: 30208932] (IF/IHC)
• Kawaguchi N, Tashiro K, Taniguchi K et al. Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells Biochim. Biophys. Acta 2018-04-20 [PMID: 29684585] (IF/IHC, WB, Human)
• Jin Y, Hu W, Liu T et al. Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel. Cancer Lett. 2018-04-10 [PMID: 29373839] (Human)
• Rodriguez-Feo JA, Gallego-Delgado J, Puerto M et al. Reticulon-4B/Nogo-B acts as a molecular linker between microtubules and actin cytoskeleton in vascular smooth muscle cells. Biochim. Biophys. Acta. 2016-04-28 [PMID: 27132996] (ICC/IF, WB, Rat)
• Teng RJ, Rana U, Afolayan AJ et al. Nogo-B Receptor Modulates Angiogenesis Response of Pulmonary Artery Endothelial Cells Through eNOS Coupling. Am. J. Respir. Cell Mol. Biol. 2014-02-25 [PMID: 24568601] (IHC-P, WB, Sheep)
  
  Details:
  IHC-P (lung), Fig 1A-D; WB [lung, pulmonary artery endothelial cells (PAEC)], Fig 1E-F. For IHC-P, the Nogo pAb was used at 1:4000. Two bands were observed in WB for Nogo in both lung and PAEC, similar to previous reports in the literature. These bands we
• Jozsef L, Tashiro K, Kuo A et al. Reticulon 4 is necessary for ER tubulation, STIM1-Orai1 coupling and store-operated calcium entry. J. Biol. Chem. 2014-03-11 [PMID: 24558039] (WB, ICC/IF, Mouse)
  
  Details:
  Mouse embryo fibroblasts (MEFs) including wildytpe (WT), knockout (kO) and RTN4b stably transfected: WB (Figs 1A, 1C, 1D; 7A, 8A, 8D), IF (Figs 1B, 12A)Note: The specificity of the pAb was validated in RTN-kO versus wildtype MEFs by WB, and in RTN4b stabl
• Chan RT, Russell RA, Marcal H et al. BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration. Biomacromolecules 2014-01-13 [PMID: 24299034] (WB, Rat)
• Wang B, Zhao B, North P et al. Expression of NgBR is highly associated with estrogen receptor alpha and survivin in breast cancer. PLoS One 2013-01-01 [PMID: 24223763] (Rabbit)
• Gramolini AO, Kislinger T, Alikhani-Koopaei R et al. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008-03-01 [PMID: 18056057] (WB, Mouse)
  
  Details:
  WB, mouse heart tissue: 1. IMG- 5346A (Nogo A/B): Fig. C. 2. IMG-3374 (FHL1): Figs. 3C; 4A, B; 6; 8A, B.
• Rodriguez-Feo JA, Hellings WE, Verhoeven BA et al. Low levels of Nogo-B in human carotid atherosclerotic plaques are associated with an atheromatous phenotype, restenosis, and stenosis severity. Arterioscler Thromb Vasc Biol. 2007-06-01 [PMID: 17413036] (ELISA, Human)
  
  Details:
  Nogo-A/B (IMG-5346A). ELISA: Human plasma, Fig 3.

Bioinformatics

• Gene Symbol: RTN4
• Entrez:
  • 57142(Human)
  • 68585(Mouse)
  • 83765(Rat)
• Uniprot:
  • Q9NQC3()