Monkey Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

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Standard Curve Reference.
Samples were spiked with high concentrations of Monkey FASN, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Samples were spiked with high concentrations of Monkey Fatty Acid Synthase/FASN, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

Monkey Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) Summary

Description
Assay Length: 3 h 30 min
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FASN

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Monkey Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

  • EC 2.3.1
  • EC 2.3.1.85
  • EC 6.3.3.1
  • FASN
  • FASshort chain dehydrogenase/reductase family 27X, member 1
  • Fatty Acid Synthase
  • MGC14367
  • MGC15706
  • OA-519
  • SDR27X1

Background

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Fatty Acid Synthase/FASN ELISA Kit (NBP3-49707). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol FASN