Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

ELISA: Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) [NBP2-82512] - Samples were spiked with high concentrations of Human Fatty Acid Synthase/FASN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) [NBP2-82512] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Fatty Acid Synthase/FASN in samples. No Significant cross-reactivity or interference between Human Fatty Acid Synthase/FASN and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.8
• Intra-Assay: CV% < 4.66
• Spike Recovery: 86-100%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FASN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

• EC 2.3.1
• EC 2.3.1.85
• EC 6.3.3.1
• FASN
• FASshort chain dehydrogenase/reductase family 27X, member 1
• Fatty Acid Synthase
• MGC14367
• MGC15706
• OA-519
• SDR27X1

Background

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Fatty Acid Synthase/FASN ELISA Kit (NBP2-82512)(1)

Publication using NBP2-82512

• Michele Dei Cas, Chiara Maura Ciniselli, Elisabetta Vergani, Emilio Ciusani, Mariachiara Aloisi, Valeria Duroni, Paolo Verderio, Riccardo Ghidoni, Rita Paroni, Paola Perego, Giovanni Luca Beretta, Laura Gatti, Monica Rodolfo, Alexander N. Orekhov, Vasily Nikolaevich Sukhorukov Alterations in Plasma Lipid Profiles Associated with Melanoma and Therapy Resistance International Journal of Molecular Sciences 2024-01-26 [PMID: 38338838]

FAQs for Fatty Acid Synthase/FASN ELISA Kit (NBP2-82512). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FASN