Fatty Acid Synthase/FASN Antibody (3F2-1F3)

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Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M01), clone 3F2-1F3.Lane 1: FASN transfected lysate(48.3 ...read more
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of monoclonal antibody to FASN on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of monoclonal antibody to FASN on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration ...read more
Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - FASN monoclonal antibody (M01), clone 3F2-1F3 Analysis of FASN expression in 293.
Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN transfected lysate using anti-FASN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FASN MaxPab ...read more
Sandwich ELISA Capture: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Detection limit for recombinant GST tagged Fatty Acid Synthase/FASN is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, ELISA(Cap)
Clone
3F2-1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Fatty Acid Synthase/FASN Antibody (3F2-1F3) Summary

Immunogen
FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
Specificity
FASN - fatty acid synthase (3F2-1F3)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FASN
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Sandwich ELISA Capture
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.
Control
Fatty Acid Synthase/FASN Knockout 293T Cell Lysate
Publications
Read Publications using
H00002194-M01 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Ascites

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fatty Acid Synthase/FASN Antibody (3F2-1F3)

  • EC 2.3.1
  • EC 2.3.1.85
  • EC 6.3.3.1
  • FASN
  • FASshort chain dehydrogenase/reductase family 27X, member 1
  • Fatty Acid Synthase
  • MGC14367
  • MGC15706
  • OA-519
  • SDR27X1

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00002194-M01
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, ELISA(Cap)

Publications for Fatty Acid Synthase/FASN Antibody (H00002194-M01)(4)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FASN
OMIM