FAM8A1 Antibody

Western Blot: FAM8A1 Antibody [NBP2-47125] - Western Blot Image of anti-FAM8A1. Total protein from human brain (lane 1), skeletal muscle (lane 2), mouse brain (lane 3) and rat testis (lane 4) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM8A1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: FAM8A1 Antibody [NBP2-47125] - Hek293 cells were fixed for 5 minute using 10% formalin and then permeabilized for 10 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-FAM8A1 at a 1:500 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: FAM8A1 Antibody [NBP2-47125] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse testes using FAM8A1 antibody at 1:500 dilution. The antibody generated a cytoplasmic staining with intense brown dots (potentially the staining of the ER) in each of the primary and secondary spermatocytic cells.

Immunohistochemistry-Paraffin: FAM8A1 Antibody [NBP2-47125] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse testes using FAM8A1 antibody at 1:200 dilution. The antibody generated a cytoplasmic staining with intense brown dots (potentially the staining of the ER) in each of the primary and secondary spermatocytic cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FAM8A1 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human FAM8A1 protein (between amino acids 50-100) [Uniprot Q9UBU6]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAM8A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 - 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAM8A1
• Uniprot:
  • Q9UBU6(Human)