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FAM8A1 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47125AF350
Alexa Fluor 405
NBP2-47125AF405
Alexa Fluor 488
NBP2-47125AF488
Alexa Fluor 532
NBP2-47125AF532
Alexa Fluor 594
NBP2-47125AF594
Alexa Fluor 647
NBP2-47125AF647
Alexa Fluor 700
NBP2-47125AF700
Alexa Fluor 750
NBP2-47125AF750
Biotin
NBP2-47125B
DyLight 350
NBP2-47125UV
DyLight 405
NBP2-47125V
DyLight 488
NBP2-47125G
DyLight 550
NBP2-47125R
DyLight 594
NBP2-47125DL594
DyLight 680
NBP2-47125FR
DyLight 755
NBP2-47125IR
FITC
NBP2-47125F
HRP
NBP2-47125H
Janelia Fluor 549
NBP2-47125JF549
Janelia Fluor 646
NBP2-47125JF646
Unconjugated
NBP2-47125
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-47125
BSA Free
NBP2-47125SS
BSA Free
NBP2-47125
100% Guarantee on every product

FAM8A1 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to an internal portion of the human FAM8A1 protein (between amino acids 50-100) [Uniprot Q9UBU6]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAM8A1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FAM8A1 Antibody [DyLight 650]

  • AHCP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line,SK‑Mel‑28 human malignant melanoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human14‑3‑3 zeta  Monoclonal Antibody (Catalog # MAB2669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for14‑3‑3 zeta at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
14‑3‑3 zeta  was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
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14-3-3 Antibody
NBP2-27202
Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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Species: Ca, Ch, ChHa, Fe, Hu, Mu, Ma-Op, Po, Pm, Rt
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CAMP/LL37/FALL39/Cathe ...
NBP1-76864
Immunohistochemistry-Paraffin: CAMP/LL37/FALL39/Cathelicidin Antibody [NBP1-76864] - IHC analysis of a formalin fixed paraffin embedded tissue section of human lymph node using 5 ug/ml dilution of CAMP antibody (NBP1-76864). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.
Western Blot: CAMP/LL37/FALL39/Cathelicidin Antibody [NBP1-76864] - Human spleen tissue lysate with Cathelicidin antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: CAMP/LL37/FALL39/Cathelicidin Antibody [NBP1-76864] - in Human Spleen cells.
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
GGT1 Antibody (1F9)
H00002678-M01
Western Blot: GGT1 Antibody (1F9) [H00002678-M01] - GGT1 monoclonal antibody (M01), clone 1F9 Analysis of GGT1 expression in NIH/3T3.
Immunohistochemistry-Paraffin: GGT1 Antibody (1F9) [H00002678-M01] - Analysis of monoclonal antibody to GGT1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Immunoprecipitation: GGT1 Antibody (1F9) [H00002678-M01] - Analysis of GGT1 transfected lysate using anti-GGT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GGT1 MaxPab rabbit polyclonal antibody.
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Species: Hu, Mu, Ye
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p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
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Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
p15INK4b/CDKN2B
Antibody (OTI3B6)
NBP2-45603
Flow Cytometry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by CDKN2B antibody.
Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Human pancreas tissue.
Immunohistochemistry: p15INK4b/CDKN2B Antibody (OTI3B6) [NBP2-45603] - Analysis of Carcinoma of Human thyroid tissue.
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HHAT Antibody
NBP1-69465
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Western Blot: HHAT Antibody [NBP1-69465] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml.
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Seladin 1 Antibody
NBP1-32247
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Western Blot: Seladin 1 Antibody [NBP1-32247] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Seladin 1 Antibody [NBP1-32247] - Seladin 1 antibody detects Seladin 1 protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with Seladin 1 antibody diluted by 1:500
(1 Publication)
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Applications: ICC/IF, IHC, IHC-P, WB
TIGD7 Antibody
NBP1-80790
Immunocytochemistry/Immunofluorescence: TIGD7 Antibody [NBP1-80790] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry: TIGD7 Antibody [NBP1-80790] - Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
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NBP1-92581
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Nup153 Antibody
NB100-93329
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Recombinant Human
CNTNAP5 GST (N-Term) ...
H00129684-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
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SOX4 Antibody
(CL5634)
NBP2-61420
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Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
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Hippocalcin
Antibody
H00003208-B01P
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Immunocytochemistry/Immunofluorescence: Hippocalcin Antibody [H00003208-B01P] - Analysis of purified antibody to HPCA on HeLa cell. (antibody concentration 10 ug/ml)
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NBP1-85511
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GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human kidney shows high expression.
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TopBP1 Antibody
NB100-217
Western Blot: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: TopBP1 Antibody [NB100-217] - Detection of mouse TopBP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TopBP1 antibody NB100-217 (lot NB100-217-2) used for IP at 3 ug per reaction. TopBP1 was also immunoprecipitated by a previous lot of this antibody (lot A300-111A-1). For blotting immunoprecipitated TopBP1, A300-111A was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Antibody
NBP1-87377
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Western Blot: Teashirt homolog 1 Antibody [NBP1-87377] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-574
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FAM8A1 Antibody
[DyLight 650]
NBP2-47125C
FAM8A1 Antibody [DyLight 650]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol FAM8A1