Factor XIIIa Antibody (AC-1A1)

Immunohistochemistry-Paraffin: Factor XIIIa Antibody (AC-1A1) [NB100-696] - Formalin fixed paraffin embedded human placenta stained with Factor XIII-A antibody (NB100-696).

• Reactivity: Hu
• Applications: IHC
• Clone: AC-1A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Factor XIIIa Antibody (AC-1A1) Summary

• Immunogen: BALB/C mice were injected with recombinant human protein corresponding to A-subunit of coagulation Factor XIII.
• Localization: Cytoplasmic and nuclear
• Marker: Dendritic Cell Marker
• Specificity: This is specific to 160 kDa protein known as Factor XIIIA.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: F13A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Paraffin 1:25-1:50
• Application Notes: IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.
• Control:

  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Factor XIIIa Antibody (AC-1A1)

• bA525O21.1 (coagulation factor XIII, A1 polypeptide)
• coagulation factor XIII A chain
• coagulation factor XIII, A1 polypeptide
• Coagulation factor XIIIa
• EC 2.3.2.13
• F13A
• F13A1
• F13Acoagulation factor XIII, A polypeptide
• factor XIIIa
• fibrin stabilizing factor, A subunit
• fibrinoligase
• FSF, A Subunit
• Protein-glutamine gamma-glutamyltransferase A chain
• TGase
• Transglutaminase A chain
• transglutaminase. plasma

Background

Factor XIII is a beta globulin found in plasma and is composed of two subunits. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Factor XIII A is the catalytic subunit and is a dimmer of molecular weight 160kDa. The B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIII A, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Factor XIII A is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F13A1
• Entrez:
  • 2162(Human)
• Uniprot:
  • P00488(Human)