Transglutaminase 2/TGM2 Antibody

Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of extracts from K562 cells using Transglutaminase 2 antibody at 1:100.

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of Transglutaminase 2/TGM2 antibody (NBP2-24470). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Analysis of Transglutaminase 2 in A) BxPC-3 cell lysate using NBP2-24470 at 2.0 ug/ml and B) MCF-7 negative control cell lysate.

Simple Western: Transglutaminase 2/TGM2 Antibody [NBP2-24470] - Simple Western lane view shows a specific band for Transglutaminase 2/TGM2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Pm, Pm
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Transglutaminase 2/TGM2 Antibody Summary

• Description: Novus Biologicals Rabbit Transglutaminase 2/TGM2 Antibody (NBP2-24470) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-Transglutaminase 2/TGM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 600-650 of human Transglutaminase 2 was used as the immunogen.
• Predicted Species: Chimpanzee (93%), Monkey (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 1:50
  • Western Blot 2-5 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 76 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Transglutaminase 2/TGM2 Antibody

• C polypeptide
• EC 2.3.2.13
• G-ALPHA-h
• protein-glutamine gamma-glutamyltransferase 2
• protein-glutamine-gamma-glutamyltransferase
• TG(C)
• TG2
• TGase C
• TGase H
• TGase-2
• TGase-H
• TGC
• TGCGNAH
• TGM2
• Tissue transglutaminase
• transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Transglutaminase 2
• Transglutaminase C
• Transglutaminase H
• transglutaminase-2
• tTG

Background

Transglutaminase 2 is historically a member of the transglutaminase family that catalyzes a calcium-dependent acyl transfer reaction between peptide bound glutamine and a suitable primary amine.The protein is known to participate in all-trans-retinoic acid mediated signaling events as a G-protein and transamidate targets such as RhoA and the retinoblastoma gene product. The action of transglutaminase 2 stabilizes proteins by conferring mechanical resistance and protection from proteolysis. The protein is expressed intracellularly and secreted on the cell surface where it interacts with proteins in the extracellular matrix and aids in anchoring cells to basement membranes. Recently, transglutaminase 2 has been identified as an important factor that interacts with fibronectin in the extracellular matrix at the cell membrane and provides survival signals and inhibits anoikis in fibroblasts. The protein is expressed in human endothelial cells. The human transglutaminase 2 gene is localized in the chromosomal region 20q12. Transcript variants encoding two different isoforms have been found for this gene. Deficiency of the protein results in early-onset diabetes type 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 2/TGM2 Antibody (NBP2-24470)(1)

Publication using NBP2-24470

• Anjali A. Satoskar, John P. Shapiro, Mikayla Jones, Cherri Bott, Samir V. Parikh, Sergey V. Brodsky, Lianbo Yu, Haikady N. Nagaraja, Daniel W. Wilkey, Michael L. Merchant, Jon B. Klein, Tibor Nadasdy, Brad H. Rovin Differentiating Staphylococcus infection-associated glomerulonephritis and primary IgA nephropathy: a mass spectrometry-based exploratory study Scientific Reports 2020-10-14 [PMID: 33057112]

Bioinformatics

• Gene Symbol: TGM2
• Uniprot:
  • P21980()