F4/80 Antibody (521204) [Unconjugated]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).

F4/80 was detected in unfixed live cells fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 521204
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

F4/80 Antibody (521204) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
  Gln28-Gly931
  Accession # Q61549
• Specificity: Detects mouse F4/80/EMR1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: EMR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for F4/80 Antibody (521204) [Unconjugated]

• ADGRE1
• cell surface glycoprotein F4/80
• DD7A5-
• DD7A5-7
• EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• EGF-like module receptor 1
• EGF-like module-containing mucin-like hormone receptor-like 1
• EGF-TM7
• Emr
• EMR1 hormone receptor
• Emr1
• F4/80
• Gpf480
• Ly7
• Ly71
• lymphocyte antigen 71
• Mouse EMR1
• Rat EMR1
• TM7L
• TM7LN3

Background

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for F4/80 Antibody (MAB5580)(17)

Publications using MAB5580

• Y Zhang, R Sang, J Bao, Z Jiang, D Qian, Y Zhou, W Su, J Wei, L Zhao, Z Wei, Y Zhao, M Shi, G Chen Schwann cell-derived CXCL2 contributes to cancer pain by modulating macrophage infiltration in a mouse breast cancer model Brain, Behavior, and Immunity, 2023-02-06;0(0):. 2023-02-06 [PMID: 36754246] (IHC, Mouse)
• MM Xu, JY Kang, S Ji, YY Wei, SL Wei, JJ Ye, YG Wang, JL Shen, HM Wu, GH Fei Melatonin Suppresses Macrophage M1 Polarization and ROS-Mediated Pyroptosis via Activating ApoE/LDLR Pathway in Influenza A-Induced Acute Lung Injury Oxidative Medicine and Cellular Longevity, 2022-11-15;2022(0):2520348. 2022-11-15 [PMID: 36425057] (ICC, Mouse)
• MS Lee, J Jeon, S Park, J Lim, HS Yang Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation Oncogene, 2022-06-16;20(0):368-380. 2022-06-16 [PMID: 35784638] (IHC, Mouse)
• L Zhang, J Chen, L Yan, Q He, H Xie, M Chen Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction Frontiers in Pharmacology, 2021-06-10;12(0):646240. 2021-06-10 [PMID: 34177571] (IHC, Mouse)
• J Ye, Y Wang, Y Xu, Z Wang, L Liu, M Wang, D Ye, J Zhang, Z Yang, Y Lin, Q Ji, J Wan Interleukin-22 deficiency alleviates doxorubicin-induced oxidative stress and cardiac injury via the p38 MAPK/macrophage/Fizz3 axis in mice Redox Biol, 2020-07-07;36(0):101636. 2020-07-07 [PMID: 32863209] (IHC, Mouse)
• A Abbadi, J Loftis, A Wang, M Yu, Y Wang, S Shakya, X Li, EV Maytin, VC Hascall Heparin inhibits pro-inflammatory and promotes anti-inflammatory macrophage polarization under hyperglycemic stress J. Biol. Chem., 2020-02-27;0(0):. 2020-02-27 [PMID: 32107314] (ICC, Mouse)
• H Tanaka, K Horioka, M Yamamoto, M Asari, K Okuda, K Yamazaki, K Shimizu, K Ogawa Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis Cancer Sci., 2019-07-30;110(9):2748-2759. 2019-07-30 [PMID: 31301081] (IHC-P, Mouse)
• E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou Osteopontin in the host response to Leishmania amazonensis BMC Microbiol., 2019-02-08;19(1):32. 2019-02-08 [PMID: 30736736] (IHC-P, Mouse)
• A Than, C Liu, H Chang, PK Duong, CMG Cheung, C Xu, X Wang, P Chen Self-implantable double-layered micro-drug-reservoirs for efficient and controlled ocular drug delivery Nat Commun, 2018-11-06;9(1):4433. 2018-11-06 [PMID: 30401883] (IHC, Mouse)
• J Zhou, J Li, Y Yu, Y Liu, H Li, Y Liu, J Wang, L Zhang, X Lu, Z Chen, D Zuo Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment J. Leukoc. Biol., 2018-10-23;0(0):. 2018-10-23 [PMID: 30351498] (Flow Cytometry, Mouse)
• L Zhuang, Y Jang, YK Park, JE Lee, S Jain, E Froimchuk, A Broun, C Liu, O Gavrilova, K Ge Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function Nat Commun, 2018-05-04;9(1):1796. 2018-05-04 [PMID: 29728617] (IHC, Mouse)
• Y Li, X Huang, S Huang, H He, T Lei, F Saaoud, XQ Yu, A Melnick, A Kumar, CJ Papasian, D Fan, M Fu Central role of myeloid MCPIP1 in protecting against LPS-induced inflammation and lung injury Signal Transduct Target Ther, 2017-12-08;2(0):17066. 2017-12-08 [PMID: 29263935] (IHC-P, Mouse)
• Maria Fe Lanfran, David J Loane, Italo Mocchetti, Mark P Burns, Sonia Villapol Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells. Bio-Protocol, 2017-11-20;0(0):2331-8325. 2017-11-20 [PMID: 29238736] (IHC, Mouse)
• MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation Proc Natl Acad Sci USA, 2016-05-19;0(0):. 2016-05-19 [PMID: 27199481] (Flow Cytometry, Mouse)
• Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity. 2013-03-25;0(0):. 2013-03-25 [PMID: 23536877] (IHC-Fr, Mouse)
• Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis. J Neuroinflammation, 2012-08-10;9(0):193. 2012-08-10 [PMID: 22883932] (IHC-Fr, Mouse)
• Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012-06-13;5(0):702. 2012-06-13 [PMID: 22692452] (IHC-Fr, Mouse)

Review for F4/80 Antibody (MAB5580) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Mozhdeh Sojoodi IHC-Fr Mouse 06/05/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Liver
• Species: Mouse
• Lot: MAB5580

FAQs for F4/80 Antibody (MAB5580). (Showing 1 - 5 of 5 FAQs).

1. Are the F4/80/EMR1 antibodies validated in Simple Western?
   • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
2. Does F4/80/EMR1 antibodies come in lyophilized form?
   • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
   • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
4. Does this F4/80/EMR1 antibody come in lyophilized form?
   • Yes, we carry this F4/80 antibody in lyophilized form.
5. What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?
   • A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

Bioinformatics

• Gene Symbol: EMR1
• Uniprot:
  • Q61549()