F4/80 Antibody (521204) [Unconjugated]

Images

 
F4/80 was detected in immersion fixed frozen sections of mouse lung using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
521204
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

F4/80 Antibody (521204) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Specificity
Detects mouse F4/80/EMR1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EMR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB5580 in the following applications:

Publications
Read Publications using
MAB5580 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for F4/80 Antibody (521204) [Unconjugated]

  • ADGRE1
  • cell surface glycoprotein F4/80
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • EMR1 hormone receptor
  • F4/80
  • Gpf480
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7LN3

Background

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F4/80 Antibody (MAB5580)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow, IHC, IHC Fresh, IHC Frozen.


Filter By Application
Flow
(2)
IHC
(2)
IHC Fresh
(1)
IHC Frozen
(2)
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Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB5580 Applications Species
J Zhou, J Li, Y Yu, Y Liu, H Li, Y Liu, J Wang, L Zhang, X Lu, Z Chen, D Zuo Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30351498] (Flow,, Mouse) Flow Mouse
L Zhuang, Y Jang, YK Park, JE Lee, S Jain, E Froimchuk, A Broun, C Liu, O Gavrilova, K Ge Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function Nat Commun, 2018;9(1):1796. 2018 [PMID: 29728617] (IHC,, Mouse) IHC Mouse
Maria Fe Lanfran, David J Loane, Italo Mocchetti, Mark P Burns, Sonia Villapol Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells. Bio-Protocol, 2017;0(0):2331-8325. 2017 [PMID: 29238736] (IHC,, Mouse) IHC Mouse
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27199481] (Flow,, Mouse) Flow Mouse
Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity., 2013;0(0):. 2013 [PMID: 23536877] (IHC Frozen,, Mouse) IHC Frozen Mouse
Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis. J Neuroinflammation, 2012;9(0):193. 2012 [PMID: 22883932] (IHC Frozen,, Mouse) IHC Frozen Mouse
Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012;5(0):702. 2012 [PMID: 22692452] (IHC Fresh,, Mouse) IHC Fresh Mouse

Review for F4/80 Antibody (MAB5580) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB5580:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen F4/80 MAB5580
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 06/05/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLiver
SpeciesMouse
LotMAB5580

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for F4/80 Antibody (MAB5580). (Showing 1 - 5 of 5 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. Does this F4/80/EMR1 antibody come in lyophilized form?
    • Yes, we carry this F4/80 antibody in lyophilized form.
  5. What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?
    • A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Array Products

Array MAB5580

Bioinformatics Tool for F4/80 Antibody (MAB5580)

Discover related pathways, diseases and genes to F4/80 Antibody (MAB5580). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on F4/80.

TMEM 119 is a specific marker of microglia cells
By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/05/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol EMR1
Uniprot