EWSR1 Antibody (5H7) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
5H7
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

EWSR1 Antibody (5H7) [FITC] Summary

Immunogen
Full length recombinant human EWSR1 expressed in and purified from E. coli. [UniProt# Q01844]
Localization
Nucleus. Cytoplasm. Cell membrane. Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EWSR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This EWSR1 (5H7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EWSR1 Antibody (5H7) [FITC]

  • bK984G1.4
  • Ewing sarcoma breakpoint region 1 protein
  • Ewing sarcoma breakpoint region 1
  • Ewings sarcoma EWS-Fli1 (type 1) oncogene
  • EWS oncogene
  • EWSRNA-binding protein EWS

Background

EWSR1 (Ewing sarcoma breakpoint region 1) belongs to TET family (Tls, EWSR1, TafII) of RNA-binding proteins and it is implicated in transcriptional regulation for specific genes, mRNA splicing and transcription initiation. EWSR1 has the potential to associate with basal transcription factor TFIID and RNA polymerase II complex, and is required for proper localization of aurora B during mitosis as well as for maintaining mitotic spindle integrity. EWSR1 also associates with EP300/CREBBP as coactivator and EWSR1-EP300/CREBBP facilitates FOS activation as well as HNF4 genes activation. EWSR1 activates other transcription factors such as POU4F1 (or BRN3A, 13q13) and POU5F1 (or OCT4, 6p21) which regulate differentiation of neuronal cells. EWSR1 and CCNL1 are also interacting partners of TFIP11 which is functionally related to spliceosome and involved in pre-mRNA splicing. EWSR1 is required for cell survival in CNS and performs important functions in regulating genomic integrity and in RNA/microRNA's maturation processes. EWSR1 is ubiquity expressed with dominant localization in the nucleus and rarely in cytoplasm or various subcellular compartments, depending on the methylation state of its RNA-binding domain. Several gene families, mainly encoding for transcriptional regulators, can translocate with EWSR1 and the chimeric proteins have an aberrant activity interfering with different molecular pathways crucial for cell growth, differentiation and proliferation. EWSR1 is involved in several sarcoma translocations and soft tissue myoepithelial tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP1-92686UV
DyLight 405 Labeled NBP1-92686V
DyLight 488 Labeled NBP1-92686G
DyLight 680 Labeled NBP1-92686FR
DyLight 755 Labeled NBP1-92686IR

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Bioinformatics

Gene Symbol EWSR1