EWSR1 Antibodies
We offer EWSR1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each EWSR1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our EWSR1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the EWSR1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our EWSR1 polyclonal antibodies and browse our EWSR1 monoclonal antibody catalog.
Alternate Names for EWSR1 Antibodies
anti-EWSR1 antibody, anti-bK984G1.4 antibody, anti-Ewing sarcoma breakpoint region 1 antibody, anti-Ewing sarcoma breakpoint region 1 protein antibody, anti-Ewings sarcoma EWS-Fli1 (type 1) oncogene antibody, anti-EWS oncogene antibody, anti-EWSRNA-binding protein EWS antibody
11 Results for "ewsr1" in Primary Antibodies
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Western-Blot-NBP1-92686-img0005.jpg "Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is clear that in these cells EWSR1 is localized along with the DNA in the nucleus.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Immunocytochemistry-Immunofluorescence-NBP1-92686-img0003.jpg)
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.](http://images.novusbio.com/fullsize/EWSR1-Antibody-5H7-Western-Blot-NBP1-92686-img0004.jpg)
![Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-182-img0018.jpg "Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.")
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NB200-182] - Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (NB200-182). Detection: DAB](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-NB200-182-img0014.jpg)
![Western Blot: EWSR1 Antibody [NB200-182] - Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-182 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-182-img0016.jpg)
![Immunoprecipitation: EWSR1 Antibody [NB200-182] - Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody NB200-182 (lot NB200-182-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot NB200-182-1). For blotting immunoprecipitated EWS, NB200-182 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunoprecipitation-NB200-182-img0015.jpg)
![Simple Western: EWSR1 Antibody [NB200-182] - Simple Western lane view shows HeLa, HEK293T, and HepG2 whole cell lysate (WCL). A specific band was detected for EWSR1 antibody (NBP1-49701) at approximately 116-140 kDa (as indicated) using 10 ug/mL of EWSR1 antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Simple-Western-NB200-182-img0017.jpg)
![Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845] - Western blot analysis of extracts of various cell lines, using EWSR1 Rabbit mAb (NBP3-16845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Western-Blot-NBP3-16845-img0007.jpg "Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845] - Western blot analysis of extracts of various cell lines, using EWSR1 Rabbit mAb (NBP3-16845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of U-2 OS cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Immunocytochemistry-Immunofluorescence-NBP3-16845-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of C6 cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Immunocytochemistry-Immunofluorescence-NBP3-16845-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of NIH-3T3 cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/EWSR1-Antibody-9G3H1-Immunocytochemistry-Immunofluorescence-NBP3-16845-img0005.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human breast cancer tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209823.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded rat colon tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-82202520982.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse kidney tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209884.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse lung tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-82202520104012.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse colon tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-8220252010463.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human thyroid tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-8220252010470.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded rat spleen tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-8220252010450.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human tonsil tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209895.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded human breast tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025209877.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse heart tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025208472.jpg)
![Immunohistochemistry: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry analysis of EWSR1 in paraffin-embedded mouse liver tissue using EWSR1 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16845_rabbit-ewsr1-mab-9g3h1-822025208433.jpg)
![Western Blot: EWSR1 Antibody [NBP2-16389] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NBP2-16389-img0004.jpg "Western Blot: EWSR1 Antibody [NBP2-16389] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody diluted at a dilution of 1:1000.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody [NBP2-16389] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16389-img0003.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse duodenum. EWSR1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-16389-img0008.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-16389-img0002.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded rat fore brain. EWSR1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-16389-img0005.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse testis. EWSR1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-16389-img0006.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse prostate. EWSR1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-16389-img0007.jpg)
![Western Blot: EWSR1 Antibody [NBP2-49380] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NBP2-49380-img0002.jpg "Western Blot: EWSR1 Antibody [NBP2-49380] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human skin.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0008.jpg)
![Immunohistochemistry: EWSR1 Antibody [NBP2-49380] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-NBP2-49380-img0001.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0003.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining in human testis and skeletal muscle tissues using anti-EWSR1 antibody. Corresponding EWSR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human colon, liver, skin and testis using Anti-EWSR1 antibody NBP2-49380 (A) shows similar protein distribution across tissues to independent antibody NBP2-49020 (B).](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0006.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human liver.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0007.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human colon.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-Paraffin-NBP2-49380-img0009.jpg)
![Western Blot: EWSR1 Antibody [NBP1-28798] - (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp1-28798_goat-polyclonal-ewsr1-antibody-imgenex-img-31193-12920231134474.jpg "Western Blot: EWSR1 Antibody [NBP1-28798] - (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/nbp1-28798_goat-polyclonal-ewsr1-antibody-imgenex-img-31193-12920231134478.jpg)
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - (7ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nbp1-28798_goat-polyclonal-ewsr1-antibody-imgenex-img-31193-129202311344713.jpg)
![Western Blot: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Western blot detection of EWSR1 in MCF7, 3T3, Hela cell lysates using EWSR1 Rabbit mAb (1:1000 diluted). Predicted band size: 69KDa. Observed band size: 85KDa.](http://images.novusbio.com/fullsize/EWSR1-Antibody-S09-3A7-Western-Blot-NBP3-14931-img0001.jpg "Western Blot: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Western blot detection of EWSR1 in MCF7, 3T3, Hela cell lysates using EWSR1 Rabbit mAb (1:1000 diluted). Predicted band size: 69KDa. Observed band size: 85KDa.")
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Immunofluorescence of EWSR1 (green) in Hela cells using NBP3-14931 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14931_rabbit-ewsr1-mab-s09-3a7-11320251555920.jpg)
![Western Blot: EWSR1 Antibody [NB200-183] - Detection of Human and Mouse EWS by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-183 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-183-img0002.jpg "Western Blot: EWSR1 Antibody [NB200-183] - Detection of Human and Mouse EWS by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-183 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EWSR1 Antibody [NB200-183] - Detection of human EWSR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWSR1 antibody NB200-183 used for IP at 6 ug per reaction. EWSR1 was also immunoprecipitated by rabbit anti-EWSR1 antibody NB200-182. For blotting immunoprecipitated EWSR1, NB200-183 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunoprecipitation-NB200-183-img0003.jpg)
![Western Blot: EWSR1 Antibody (S04-4B3) [NBP3-19628] - Western blot detection of EWSR1 in Hela cell lysates using NBP3-19628 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/EWSR1-Antibody-S04-4B3-Western-Blot-NBP3-19628-img0001.jpg "Western Blot: EWSR1 Antibody (S04-4B3) [NBP3-19628] - Western blot detection of EWSR1 in Hela cell lysates using NBP3-19628 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 85kDa.")
