EVI-1 Antibody (702321)

EVI-1 was detected in immersion fixed SK-OV-3 human ovarian adenocarcinoma cell line using Mouse Anti-Human EVI-1 Monoclonal Antibody (Catalog # MAB75061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 702321
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

EVI-1 Antibody (702321) Summary

• Immunogen: E. coli-derived recombinant human EVI-1
  Gly241-Met430
  Accession # Q03112
• Specificity: Detects human EVI-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MECOM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EVI-1 Antibody (702321)

• AML1-EVI-1 fusion protein
• AML1-EVI-1
• ecotropic viral integration site 1
• Ecotropic virus integration site 1 protein homolog
• EVI1
• EVI-1
• EVI1MDS1-EVI1
• MDS1 and EVI1 complex locus protein EVI1
• MDS1 and EVI1 complex locus protein MDS1
• MDS1 and EVI1 complex locus
• MDS1
• MECOM
• MGC163392
• MGC97004
• Myelodysplasia syndrome 1 protein
• myelodysplasia syndrome 1
• Myelodysplasia syndrome-associated protein 1
• oncogene EVI1
• PRDM3
• zinc finger protein Evi1

Background

Ectopic virus integration site 1 (EVI-1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI-1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI-1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI-1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI-1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI-1 shares 94% aa sequence identity with mouse and rat EVI-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EVI-1 Antibody (MAB75061)(3)

Publications using MAB75061

• Achieng, MA;Schnell, J;Fausto, CC;Csipán, RL;Koppitch, K;Thornton, ME;Grubbs, BH;Lindström, NO; Axial nephron fate switching demonstrates a plastic system tunable on demand Nature communications 2025-08-25 [PMID: 40855070] (Immunohistochemistry, Human)
• Achieng, MA;Schnell, J;Fausto, CC;Csipán, RL;Thornton, ME;Grubbs, BH;Lindström, NO; Axial Nephron Fate Switching Demonstrates a Plastic System Tunable on Demand bioRxiv : the preprint server for biology 2025-04-02 [PMID: 40236027] (Immunohistochemistry, Human)
• Lindstrom NO, De Sena Brandine G, Tran T et al. Progressive Recruitment of Mesenchymal Progenitors Reveals a Time-Dependent Process of Cell Fate Acquisition in Mouse and Human Nephrogenesis Dev. Cell 6/4/2018 [PMID: 29870722]

Bioinformatics

• Gene Symbol: MECOM
• Uniprot:
  • Q03112()