Recombinant Human Erythropoietin/EPO Protein

Product Discontinued

Recombinant Human Erythropoietin/EPO Protein Summary

• Description: Recombinant bioactive protein containing 166 amino acids for Human Erythropoietin
  
  

  Source:CHO cells
  Amino Acid Sequence:(P01588)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: Determined by a cell proliferation assay using TF-1 cells. The expected ED50 for this effect is 0.8-1.0 ng/ml.
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: EPO
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from sodium citrate buffer containing NaCl. Each mg of lyophilized protein contains 0.58 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers containing a carrier protein (0.1% BSA) and stored in working aliquots at -20C to -80C for long-term storage.

Alternate Names for Recombinant Human Erythropoietin/EPO Protein

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Recombinant Human Erythropoietin-alpha produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21 kDa and apparent glycosylated molecular mass of 30.4 kDa. EPO-alpha is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPO