Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free

Western Blot: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537] - Human Heart and HepG2 Cell Lysate using EPO Monoclonal Antibody

Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Erythropoietin/EPO Antibody (EPO/1368).

Protein Array: Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free [NBP2-54537] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Erythropoietin/EPO Antibody (EPO/1368).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, MA, CyTOF-ready
• Clone: EPO/1368
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53392).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 28-162) of human Erythropoietin/EPO protein (exact sequence is proprietary) (Uniprot: P01588)
• Localization: Cytoplasmic and Cell Surface
• Marker: Marker of Placentation Disorders
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPO
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 2-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 - 8 ug/ml
  • Protein Array
  • Western Blot 0.5 - 1 ug/ml
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Erythropoietin/EPO Antibody (EPO/1368) - Azide and BSA Free

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Erythropoietin (Epo) stimulates red blood cell production by docking with its cognate receptor on the erythroid progenitor cell and triggering an array of signaling pathways that inhibit apoptosis and promote cell proliferation and differentiation. In its pharmaceutical forms, epoetin and darbepoetin, Epo is widely used to treat various anemias, including those associated with cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin/EPO Antibody (NBP2-54537)(1)

Publication using NBP2-54537

• Desharnais P, Naud JF, Ayotte C Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system. Drug Test Anal. 2018-11-10 [PMID: 30365250] (WB, Human)
  
  Details:
  Citation using the Azide and BSA Free form of this antibody.

Bioinformatics

• Gene Symbol: EPO