Erythropoietin/EPO Antibody

Product Discontinued

Erythropoietin/EPO Antibody Summary

• Immunogen: Recombinant human erythropoietin expressed in CHO cells.
• Localization: Secreted
• Specificity: EPO
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: I-ELISA, a working antibody concentration of approximately 1 ug/ml detects 3 ng/well of recombinant human EPO. WB: use at a concentration of 2 ug/ml detects recombinant human EPO at 100 ng/lane under both reducing and non-reducing conditions. IHC(P): Use at a concentration of 15ug/ml. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species. Mouse reactivity reported in scientific literature (PMID: 26047733). Rat reactivity reported in scientific literature (PMID: 29137321).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Erythropoietin/EPO Antibody

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Anti-Erythropoietin is tested for its ability to neutralize the biological activity of recombinant human EPO on the human cell line TF-1. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant human EPO, when recombinant human EPO is present at a concentration just high enough to elicit a maximum response. Erythropoietin is a humoral factor that stimulates erythropoiesis. EPO has four introns and five exons and contains 165 amino acids. EPO induces growth and differentiation of burst forming units and colony forming units into mature red blood cells. Human erythropoietin stimulates growth of rodent bone marrow cells. Recombinant erythropoietin is produced in CHO cells by a DNA sequence encoding the human EPO protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin/EPO Antibody (NB600-538)(2)

Publications using NB600-538

• Wu Chih-Jen, Chen Cheng-Yi, Lai Thung-S et al. The role of indoxyl sulfate in renal anemia in patients with chronic kidney disease. Oncotarget 2017-01-01 [PMID: 29137321] (WB, Rat)
• Ryou MG, Choudhury GR, Li W et al. Methylene blue-induced neuronal protective mechanism against hypoxia-reoxygenation stress Neuroscience. 2015-06-03 [PMID: 26047733] (WB, Mouse)

Bioinformatics

• Gene Symbol: EPO
• Entrez:
  • 2056(Human)