ERR alpha/NR3B1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)

Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates

Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ERR alpha/NR3B1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ERR alpha/NR3B1 Antibody - BSA Free (NBP1-47254) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ERR alpha/NR3B1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an N-terminal portion of human Estrogen Related Receptor alpha (within residues 1-50). [Swiss-Prot# P11474]
• Localization: Nucleus, Cytoplasm
• Predicted Species: Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: This Estrogen Related Receptor alpha antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. ERR alpha/ ESRRA localize mainly to the nucleus, but there are reports on its cytoplasmic presence also, especially in cells with high nuclear expression. In a feedback loop mechanism, it has been shown that ERK8 /MAPK15 interacts with ERR alpha to induce its CRM1-dependent translocation to cytoplasm for inhibiting its transcriptional activity via regulation of its cellular localization (PMIDs 21190936, 18070081). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  Raw 264.7 Whole Cell Lysate

• Control Peptide:

  ERR alpha/NR3B1 Peptide (NBP1-47254PEP)

Reactivity Notes

Predicted to react with rat, pig and dog based on 100% sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERR alpha/NR3B1 Antibody - BSA Free

• ERR alpha
• ERR1
• ERR1Estrogen receptor-like 1
• ERRa
• ESRL1
• ESRL1ERR-alpha
• ESRRA
• estrogen-related receptor alphaERRalpha
• NR3B1
• NR3B1Nuclear receptor subfamily 3 group B member 1
• steroid hormone receptor ERR1

Background

Nuclear receptors are essential in embryonic development as well as for the regulation of adult physiological functions, and estrogen related receptor alpha (ERR-alpha) is one such receptors expressed in several tissues requiring high energy demand, including heart, skeletal muscle and brain. ERR-alpha binds to ERRE (ERR-alpha response element) containing a single consensus half-site, 5'-TNAAGGTCA-3'; to MCAD response element NRRE-1 for regulating MCAD promoter, and it also regulates lactoferrin gene promoter. ERR-alpha is an orphan nuclear hormone receptor capable of regulating transcription of genes involved in multiple cellular/physiological processes such as regulation of energy metabolism wherein it is co-activated by PPAR gamma and PGC-1 alpha to regulate several genes required for mitochondrial function. ERR-alpha regulates estrogen signaling and fatty acid oxidation/adaptative bioenergetic response, and is involved in cartilage/bone formation, macrophage activation and carcinogenesis. High ERR-alpha expression levels coupled with poor prognosis have been observed in various malignancies including colon, endometrium, ovary, prostate and breast cancers, and adrenocortical carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERR alpha/NR3B1 Antibody (NBP1-47254)(7)

Publications using NBP1-47254

• Kim M, Kim J, Moon S et al. Korean Red Ginseng Improves Astrocytic Mitochondrial Function by Upregulating HO-1-Mediated AMPK alpha-PGC-1 alpha-ERR alpha Circuit after Traumatic Brain Injury International journal of molecular sciences 2021-12-03 [PMID: 34884886] (WB, Human, Mouse)
• Wang L, Yang M, Jin H PI3K/AKT phosphorylation activates ERR alpha by upregulating PGC‑1 alpha and PGC‑1 beta in gallbladder cancer Molecular medicine reports 2021-08-01 [PMID: 34184087] (WB)
• Hauck L, Stanley-Hasnain S, Fung A et al. Cardiac-specific ablation of the E3 ubiquitin ligase Mdm2 leads to oxidative stress, broad mitochondrial deficiency and early death PLoS ONE 2017-12-21 [PMID: 29267372] (WB, Mouse)
• Mak TW, Hauck L, Grothe D, Billia F. p53 regulates the cardiac transcriptome. Proc. Natl. Acad. Sci. U.S.A. 2017-02-28 [PMID: 28193895]
• Hwang J, Purdy JG, Wu K et al. Estrogen-related receptor alpha is required for efficient human cytomegalovirus replication. Proc. Natl. Acad. Sci. U.S.A. 2014-12-30 [PMID: 25512541] (WB, Human)
• Silvennoinen M, Rinnankoski-Tuikka R et al. High-fat feeding induces angiogenesis in skeletal muscle and activates angiogenic pathways in capillaries Angiogenesis 2012-10-23 [PMID: 23090645] (WB, Human)
• Rinnankoski-Tuikka R, Silvennoinen M, Torvinen S et al. Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle Nutr Metab (Lond) 2012-06-09 [PMID: 22682013] (WB, Mouse)

Review for ERR alpha/NR3B1 Antibody (NBP1-47254) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/02/2023

Summary

• Application: Western Blot
• Sample Tested: glioblastoma cell lysates
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: ESRRA
• Entrez:
  • 2101(Human)
  • 26379(Mouse)
  • 293701(Rat)
• Uniprot:
  • P11474(Human)