M-Cadherin/Cadherin-15 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human M-Cadherin/Cadherin-15 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
M‑Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human M‑Cadherin/Cadherin-15 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4096) at 10 µg/mL for ...read more
C2C12 mouse myoblast cell line was stained with Sheep Anti-Human M‑Cadherin/Cadherin‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4096, filled histogram) or control antibody (Catalog # 5-001-A, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

M-Cadherin/Cadherin-15 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human M-Cadherin/Cadherin-15
Val22-Ala606
Accession # P55291
Specificity
Detects human M‑Cadherin/Cadherin‑15 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CDH15
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF4096 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-Cadherin/Cadherin-15 Antibody [Unconjugated]

  • cadherin 15, M-cadherin (myotubule)
  • cadherin 15, type 1, M-cadherin (myotubule)
  • Cadherin-14
  • Cadherin-15
  • cadherin-3
  • CDH14
  • CDH14cadherin-14
  • CDH15
  • CDH3cadherin-15
  • CDHM
  • MCAD
  • MCadherin
  • M-Cadherin
  • MRD3
  • Muscle cadherin
  • muscle-cadherin

Background

M-Cadherin (M-CAD or Cadherin-15) is a 124 kDa type I transmembrane glycoprotein of the Cadherin superfamily of calcium-dependent homotypic adhesion molecules (1-4). Like other classical Cadherins, the 814 amino acid (aa) human M-CAD contains a signal sequence (21 aa), a propeptide (29 aa), an extracellular domain with five Cadherin domain repeats (ECD, 556 aa), a transmembrane segment (20 aa) and a cytoplasmic domain (188 aa) (1). A splice variant that diverges within the third Cadherin repeat has been sequenced. The Cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells (1-4). Intracellularly, M-CAD binds beta -catenin or plakoglobin ( gamma -catenin), which in turn bind alpha -catenin (4). M-CAD also binds p120 catenin (5). Connection of Cadherin/catenin complexes to the actin cytoskeleton is in question, but is possible through a linker (6). Connection to microtubules has been shown (7). M-CAD is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion (8). It is also present in muscle satellite cells and participates in muscle regeneration (9). M-CAD is also expressed in the granule cell layer of the cerebellar glomerulus (10). Deletion of mouse M-CAD has little effect in vivo, most likely due to compensation by N-Cadherin (11). However, M-CAD upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion (8, 12, 13). M-CAD activity is later downregulated by sequestering to caveoli, p120 catenin/RhoA-induced ubiquitination and/or cleavage by calpain-3. This terminates fusion and allows sarcomere formation (5, 12, 13). Human M-CAD ECD shows 88% aa identity with mouse, rat, or bovine and 85% aa identity with canine M-CAD ECD. M-CAD is an outlier among classical Cadherins, with 40% aa identity or less in the ECD (4).

  1. Kaufmann, U. et al. (1999) Cell Tissue Res. 296:191.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Shimoyama, Y. et al. (1998) J. Biol. Chem. 273:10011.
  4. Kuch, C. et al. (1997) Exp. Cell Res. 232:331.
  5. Charasse, S. et al. (2006) Mol. Biol. Cell 17:749.
  6. Weis, W.I. and W. J. Nelson (2006) J. Biol. Chem. 281:35593.
  7. Kaufmann, U. et al. (1999) J. Cell Sci. 112:55.
  8. Zeschnigk, M. et al. (1995) J. Cell Sci. 108:2973.
  9. Wrobel, E. et al. (2007) Eur. J. Cell Biol. Jan 10; [Epub ahead of print]
  10. Rose, O. et al. (1995) Proc. Natl. Acad. Sci. USA 92:6022.
  11. Hollnagel, A. et al. (2002) Mol. Cell. Biol. 22:4760.
  12. Volonte, D. et al. (2003) Mol. Biol. Cell 14:4075.
  13. Kramerova, I. et al. (2006) Mol. Cell. Biol. 26:8437.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDH15
Entrez
Uniprot