ERO1L Antibody

Images

 
Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.
Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.
Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

ERO1L Antibody Summary

Immunogen
A synthetic peptide made to the N-terminal region of human ERO1L [UniProt Q96HE7]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERO1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed. Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2525 in the following applications:

Publications
Read Publications using
NB100-2525 in the following applications:

  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
BBS and 1.0% BSA
Preservative
No Preservative
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERO1L Antibody

  • EC 1.8.4
  • EC 1.8.4.-
  • Endoplasmic oxidoreductin-1-like protein
  • ERO1 (S. cerevisiae)-like
  • ERO1A
  • ERO1-alpha
  • ERO1L alpha
  • ERO1-l alpha
  • ERO1L
  • ERO1-L
  • ERO1-L-alpha
  • ERO1-like (S. cerevisiae)
  • ERO1-like alpha
  • ERO1-like protein alpha
  • ERO1-like
  • oxidoreductin-1-L-alpha

Background

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on lumenal side, its association with ERP44 being essential for its retention in the ER, and is unique in coupleing oxygen reduction to de novo disulfides formation. ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in upper digestive tract especially the esophagus, and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulphide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accept electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly P4HB/PDI isomerase through direct disulfide exchange and can associates with ERP44. Its reoxidation probably involves electron transfer to molecular oxygen via FAD and acts independently of GSH. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ERO1L Antibody (NB100-2525)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


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WB
(9)
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Human
(3)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB100-2525 Applications Species
Woehlbier U, Colombo A, Saaranen MJ et al. ALS-linked protein disulfide isomerase variants cause motor dysfunction. EMBO J. 2016 Feb 11 [PMID: 26869642] (WB, Mouse) WB Mouse
Gao XH, Krokowski D, Guan BJ et al. Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. Elife 2015 Nov 23 [PMID: 26595448] (WB) WB
Kukita K, Tamura Y, Tanaka T et al. Cancer-Associated Oxidase ERO1-alpha Regulates the Expression of MHC Class I Molecule via Oxidative Folding J. Immunol. 2015 May 15 [PMID: 25870246] (WB, Human) WB Human
Fu Z, Lofqvist CA, Shao Z et al. Dietary omega-3 polyunsaturated fatty acids decrease retinal neovascularization by adipose-endoplasmic reticulum stress reduction to increase adiponectin. Am J Clin Nutr 2015 Apr [PMID: 25833984] (WB) WB
Matus S, Lopez E, Valenzuela V et al. Functional Contribution of the Transcription Factor ATF4 to the Pathogenesis of Amyotrophic Lateral Sclerosis. PLoS One 2013 Jul 18 [PMID: 23874395] (WB, Mouse) WB Mouse
Fung KY, Ooi CC, Lewanowitsch T et al. Identification of potential pathways involved in induction of apoptosis by butyrate and 4-benzoylbutyrate in HT29 colorectal cancer cells J Proteome Res 2012 Oct 11 [PMID: 23057685] (WB, Human) WB Human
Devarajan A, Grijalva VR, Bourquard N et al. Macrophage Paraoxonase 2 regulates calcium homeostasis and cell survival under Endoplasmic Reticulum stress conditions and is sufficient to prevent the development of aggravated atherosclerosis in Paraoxonase 2 deficiency /apoE-/- mice on a Western diet Mol Genet Metab 2012 Jul 14 [PMID: 22864055] (WB, Mouse) WB Mouse
Jeschke MG, Gauglitz GG, Song J et al. Calcium and ER stress mediate hepatic apoptosis after burn injury. J Cell Mol Med 2009 Aug [PMID: 20141609] (WB, Rat) WB Rat
Hammer E, Bien S, Salazar MG et al. Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches. Proteomics 2010 Jan [PMID: 20017144] (WB, Human) WB Human

Review for ERO1L Antibody (NB100-2525) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2525:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot ERO1L NB100-2525
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reviewed by:
Anonymous
WB Mouse 10/05/2015
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Summary

ApplicationWestern Blot
Sample Testedwhite adipose tissue
SpeciesMouse

Blocking

Blocking Details1% BSA, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptiongoat anti-rabbit HRP
Secondary Concentration1:5000

Details

Detection Notesexposure time: 2-10min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERO1L Antibody (NB100-2525) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2525AF405
Alexa Fluor 488 Labeled NB100-2525AF488
Alexa Fluor 647 Labeled NB100-2525AF647
Alexa Fluor 700 Labeled NB100-2525AF700
Biotin Labeled NB100-2525B
DyLight 488 Labeled NB100-2525G
DyLight 550 Labeled NB100-2525R
DyLight 650 Labeled NB100-2525C
HRP Labeled NB100-2525H

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Bioinformatics Tool for ERO1L Antibody (NB100-2525)

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Blogs on ERO1L.

ERO1 Activity: A Potential Source of ER-Derived Oxidative Stress.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that are controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is ERO (ER oxidoreduction), a glycosylated...  Read full blog post.

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Recent Reviews

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5
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1
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Anonymous
10/05/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ERO1L
Entrez