ERO1L Antibody

Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.

Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.

Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

ERO1L Antibody Summary

• Immunogen: A synthetic peptide made to the N-terminal region of human ERO1L [UniProt Q96HE7]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERO1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
• Application Notes: This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed. Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1.0% BSA
• Preservative: No Preservative
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERO1L Antibody

• EC 1.8.4
• EC 1.8.4.-
• Endoplasmic oxidoreductin-1-like protein
• ERO1 (S. cerevisiae)-like
• ERO1A
• ERO1-alpha
• ERO1L alpha
• ERO1-l alpha
• ERO1L
• ERO1-L
• ERO1-L-alpha
• ERO1-like (S. cerevisiae)
• ERO1-like alpha
• ERO1-like protein alpha
• ERO1-like
• oxidoreductin-1-L-alpha

Background

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on lumenal side, its association with ERP44 being essential for its retention in the ER, and is unique in coupleing oxygen reduction to de novo disulfides formation. ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in upper digestive tract especially the esophagus, and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulphide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accept electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly P4HB/PDI isomerase through direct disulfide exchange and can associates with ERP44. Its reoxidation probably involves electron transfer to molecular oxygen via FAD and acts independently of GSH. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERO1L Antibody (NB100-2525)(10)

Publications using NB100-2525

• Park MJ, Iyer S, Xue X et al. HIF1-alpha Regulates Acinar Cell Function and Response to Injury in Mouse Pancreas. Gastroenterology 2018 May [PMID: 29409830]
• Woehlbier U, Colombo A, Saaranen MJ et al. ALS-linked protein disulfide isomerase variants cause motor dysfunction. EMBO J. 2016 Feb 11 [PMID: 26869642] (WB, Mouse)
• Gao XH, Krokowski D, Guan BJ et al. Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. Elife 2015 Nov 23 [PMID: 26595448] (WB)
• Kukita K, Tamura Y, Tanaka T et al. Cancer-Associated Oxidase ERO1-alpha Regulates the Expression of MHC Class I Molecule via Oxidative Folding J. Immunol. 2015 May 15 [PMID: 25870246] (WB, Human)
• Fu Z, Lofqvist CA, Shao Z et al. Dietary omega-3 polyunsaturated fatty acids decrease retinal neovascularization by adipose-endoplasmic reticulum stress reduction to increase adiponectin. Am J Clin Nutr 2015 Apr [PMID: 25833984] (WB)
• Matus S, Lopez E, Valenzuela V et al. Functional Contribution of the Transcription Factor ATF4 to the Pathogenesis of Amyotrophic Lateral Sclerosis. PLoS One 2013 Jul 18 [PMID: 23874395] (WB, Mouse)
• Fung KY, Ooi CC, Lewanowitsch T et al. Identification of potential pathways involved in induction of apoptosis by butyrate and 4-benzoylbutyrate in HT29 colorectal cancer cells J Proteome Res 2012 Oct 11 [PMID: 23057685] (WB, Human)
• Devarajan A, Grijalva VR, Bourquard N et al. Macrophage Paraoxonase 2 regulates calcium homeostasis and cell survival under Endoplasmic Reticulum stress conditions and is sufficient to prevent the development of aggravated atherosclerosis in Paraoxonase 2 deficiency /apoE-/- mice on a Western diet Mol Genet Metab 2012 Jul 14 [PMID: 22864055] (WB, Mouse)
• Jeschke MG, Gauglitz GG, Song J et al. Calcium and ER stress mediate hepatic apoptosis after burn injury. J Cell Mol Med 2009 Aug [PMID: 20141609] (WB, Rat)
• Hammer E, Bien S, Salazar MG et al. Proteomic analysis of doxorubicin-induced changes in the proteome of HepG2cells combining 2-D DIGE and LC-MS/MS approaches. Proteomics 2010 Jan [PMID: 20017144] (WB, Human)

Review for ERO1L Antibody (NB100-2525) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 10/05/2015

Summary

• Application: Western Blot
• Sample Tested: white adipose tissue
• Species: Mouse

Blocking

• Blocking Details: 1% BSA, 1 hour, room temperature

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: goat anti-rabbit HRP
• Secondary Concentration: 1:5000

Details

• Detection Notes: exposure time: 2-10min

Bioinformatics

• Gene Symbol: ERO1L