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ERO1L Antibody

 (11 Publications)
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Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.
Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.
Immunocytochemistry/ Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.
Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.
Western Blot: ERO1L Antibody [NB100-2525] - Thermal injury induces hepatic endoplasmic reticulum (ER) stress response in vivo. Western blots of ER stress proteins and ER calcium handling/chaperone proteins and/or their ...read more
Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2525AF350
Alexa Fluor 405
NB100-2525AF405
Alexa Fluor 488
NB100-2525AF488
Alexa Fluor 532
NB100-2525AF532
Alexa Fluor 594
NB100-2525AF594
Alexa Fluor 647
NB100-2525AF647
Alexa Fluor 700
NB100-2525AF700
Alexa Fluor 750
NB100-2525AF750
Biotin
NB100-2525B-100ul
DyLight 350
NB100-2525UV
DyLight 405
NB100-2525V
DyLight 488
NB100-2525G-100ul
DyLight 550
NB100-2525R-100ul
DyLight 594
NB100-2525DL594
DyLight 650
NB100-2525C-100ul
DyLight 680
NB100-2525FR
DyLight 755
NB100-2525IR
FITC
NB100-2525F
HRP
NB100-2525H
Janelia Fluor 549
NB100-2525JF549
Janelia Fluor 646
NB100-2525JF646
100% Guarantee on every product

ERO1L Antibody Summary

Immunogen
A synthetic peptide made to the N-terminal region of human ERO1L [UniProt Q96HE7]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERO1A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed. Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-2525 in the following applications:
Publications
Read Publications using
NB100-2525 in the following applications:

  • WB
    10 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34512174). .

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 1.0% BSA
Preservative
No Preservative
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERO1L Antibody

  • EC 1.8.4
  • EC 1.8.4.-
  • Endoplasmic oxidoreductin-1-like protein
  • ERO1 (S. cerevisiae)-like
  • ERO1A
  • ERO1-alpha
  • ERO1L alpha
  • ERO1-l alpha
  • ERO1L
  • ERO1-L
  • ERO1-L-alpha
  • ERO1-like (S. cerevisiae)
  • ERO1-like alpha
  • ERO1-like protein alpha
  • ERO1-like
  • oxidoreductin-1-L-alpha

Background

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on lumenal side, its association with ERP44 being essential for its retention in the ER, and is unique in coupleing oxygen reduction to de novo disulfides formation. ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in upper digestive tract especially the esophagus, and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulphide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accept electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly P4HB/PDI isomerase through direct disulfide exchange and can associates with ERP44. Its reoxidation probably involves electron transfer to molecular oxygen via FAD and acts independently of GSH. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes.
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Immunohistochemistry-Paraffin: PADI1 Antibody [NBP2-37761] - Staining of human colon shows low expression as expected.
Immunohistochemistry-Paraffin: PADI1 Antibody [NBP2-37761] - Staining of human esophagus shows cytoplasmic and nuclear positivity in squamous epithelial cells.
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Immunohistochemistry-Paraffin: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Immunohistochemistry images are showing a stronger cytoplasmic staining of ERO1LB in (A) primary colorectal cancer compared to (B) normal colon mucosa using ERO1LB Antibody (M37-P5D11). IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
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(4 Publications)
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p53 Antibody (PAb
240) - BSA Free
NB200-103
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
(1 Review)
(2 Publications)
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Applications: ICC/IF, IHC, IHC-P, IP, WB
ERO1L Antibody
NB100-2525
ERO1L Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF

Publications for ERO1L Antibody (NB100-2525)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-2525 Applications Species
Liu Y, Hu R, Shen H et al. Endophilin A2-mediated alleviation of endoplasmic reticulum stress-induced cardiac injury involves the suppression of ERO1 alpha /IP3R signaling pathway International journal of biological sciences Aug 26 2021 [PMID: 34512174] (WB, Rat) WB Rat
Park MJ, Iyer S, Xue X et al. HIF1-alpha Regulates Acinar Cell Function and Response to Injury in Mouse Pancreas. Gastroenterology 2018 May [PMID: 29409830]
Woehlbier U, Colombo A, Saaranen MJ et al. ALS-linked protein disulfide isomerase variants cause motor dysfunction. EMBO J. 2016 Feb 11 [PMID: 26869642] (WB, Mouse) WB Mouse
Gao XH, Krokowski D, Guan BJ et al. Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. Elife 2015 Nov 23 [PMID: 26595448] (WB) WB
Kukita K, Tamura Y, Tanaka T et al. Cancer-Associated Oxidase ERO1-alpha Regulates the Expression of MHC Class I Molecule via Oxidative Folding J. Immunol. 2015 May 15 [PMID: 25870246] (WB, Human) WB Human
Fu Z, Lofqvist CA, Shao Z et al. Dietary omega-3 polyunsaturated fatty acids decrease retinal neovascularization by adipose-endoplasmic reticulum stress reduction to increase adiponectin. Am J Clin Nutr 2015 Apr [PMID: 25833984] (WB) WB
Matus S, Lopez E, Valenzuela V et al. Functional Contribution of the Transcription Factor ATF4 to the Pathogenesis of Amyotrophic Lateral Sclerosis. PLoS One 2013 Jul 18 [PMID: 23874395] (WB, Mouse) WB Mouse
Fung KY, Ooi CC, Lewanowitsch T et al. Identification of potential pathways involved in induction of apoptosis by butyrate and 4-benzoylbutyrate in HT29 colorectal cancer cells J Proteome Res 2012 Oct 11 [PMID: 23057685] (WB, Human) WB Human
Devarajan A, Grijalva VR, Bourquard N et al. Macrophage Paraoxonase 2 regulates calcium homeostasis and cell survival under Endoplasmic Reticulum stress conditions and is sufficient to prevent the development of aggravated atherosclerosis in Paraoxonase 2 deficiency /apoE-/- mice on a Western diet Mol Genet Metab 2012 Jul 14 [PMID: 22864055] (WB, Mouse) WB Mouse
Jeschke MG, Gauglitz GG, Song J et al. Calcium and ER stress mediate hepatic apoptosis after burn injury. J Cell Mol Med 2009 Aug [PMID: 20141609] (WB, Rat) WB Rat
Show All 11 Publications.

Review for ERO1L Antibody (NB100-2525) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2525:
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Western Blot ERO1L NB100-2525
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reviewed by:
ZHONGJIE FU
 WB Mouse 10/05/2015
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Summary

ApplicationWestern Blot
Sample Testedwhite adipose tissue
SpeciesMouse

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Secondary Antibodies

 

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ERO1 Activity: A Potential Source of ER-Derived Oxidative Stress.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that are controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is ERO (ER oxidoreduction), a glycosylated...  Read full blog post.
Omicron Variant Antibodies

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Recent Reviews

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Western Blot ERO1L NB100-2525
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 ZHONGJIE FU
10/05/2015
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol ERO1A