IP3R1 Antibody

Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.

Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).

Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

IP3R1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2708 and 2758 of human inositol 1,4,5-trisphosphate receptor type 1 using the numbering given in entry Q14643.2 (GeneID 3708).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITPR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IP3R1 Antibody

• DKFZp313E1334
• DKFZp313N1434
• inositol 14,5-triphosphate receptor, type 1
• inositol 14,5-trisphosphate receptor type 1
• InsP3R1
• IP3 receptor isoform 1
• IP3 receptor
• IP3R 1
• IP3R
• IP3R1
• ITPR1
• SCA15
• SCA16
• spinocerebellar ataxia 15
• spinocerebellar ataxia 16
• Type 1 inositol 14,5-trisphosphate receptor
• Type 1 InsP3 receptor

Background

IP3R1 (inositol 1,4,5-triphosphate receptor type 1) is an intracellular calcium release channel that mediates calcium release from the endoplasmic reticulum. There are 3 mammalian InsP3R receptors: Insp3R1, Insp3R2, and Insp3R3. Defects in the gene which encodes InsP3R1, ITPR1, are the cause of spinocerebellar ataxia type 15 (SCA15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IP3R1 Antibody (NBP1-21398)(2)

Publications using NBP1-21398

• Suriano CM. Inositol 1,4,5-triphosphate receptor is selectively expressed in cerebellum but not cerebellum-like structures of the elasmobranch fish, Leucoraja erinacea Heliyon 2019-01-01 [PMID: 30775573] (WB, Mouse)
• Wang Y, Li G, Goode J et al. Inositol-1,4,5-trisphosphate receptor regulates hepatic gluconeogenesis in fasting and diabetes. Nature 2012-04-01 [PMID: 22495310]

Review for IP3R1 Antibody (NBP1-21398) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 04/20/2016

Summary

• Application: Western Blot
• Sample Tested: Hindbrain
• Species: Other

Bioinformatics

• Gene Symbol: ITPR1
• Entrez:
  • 3708(Human)
• Uniprot:
  • Q14643.2(Human)