ER alpha/NR3A1 [p Ser106] Antibody

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NB100-81912. The picture on the right is blocked with the phospho peptide.

Western Blot: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912] - Western blot analysis of lysates from MCF7 cells, using NB100-81912. The lane on the left is blocked with the phospho peptide

Western Blot: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912] - Western Blot analysis of RAT-BRAIN cells using NB100-81912 diluted at 1:500

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ER alpha/NR3A1 [p Ser106] Antibody Summary

• Description: Novus Biologicals Rabbit ER alpha/NR3A1 [p Ser106] Antibody (NB100-81912) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ER alpha/NR3A1 around the phosphorylation site of serine 106 (S-P- SP-P-L).
• Modification: p Ser106
• Specificity: Detects endogenous levels of ER alpha/NR3A1 only when phosphorylated at serine 106.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ER alpha/NR3A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ER alpha/NR3A1 [p Ser106] Antibody

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1
• Entrez:
  • 2099(Human)
  • 13982(Mouse)
• Uniprot:
  • P03372()