EPS15 Antibody - BSA Free

Western Blot: EPS15 Antibody [NBP3-12544] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544] - Staining of human EPS15 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544] - Staining of human EPS15 in human stomach with rabbit polyclonal antibody at 1:500 dilution.

Immunoprecipitation: EPS15 Antibody [NBP3-12544] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-EPS15 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EPS15 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :150 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EPS15 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human EPS15 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPS15
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for EPS15 Antibody - BSA Free

• AF1P
• AF-1P
• ALL1 fused gene from chromosome 1
• epidermal growth factor receptor pathway substrate 15
• epidermal growth factor receptor substrate 15
• Eps15
• MLLT5
• Protein AF-1p
• Protein Eps15

Background

EPS15 is a ubiquitously expressed protein that is involved in cell growth regulation and may be involved in the regulation of mitogenic signals and control of cell proliferation. EPS15 may be phosphorylated on tyrosine 849 following DNA damage, resulting in the internalization of EGFR. A chromosomal aberration involving EPS15 is also found in acute myelogeneous leukemias, resulting in a rogue activator protein, so EPS15 antibodies may be useful for cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPS15