Dynamin 2 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.
Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.
Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Dynamin 2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PPQIPSRPVRIPPGIPPGVPSRR
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockout Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Knockout validation (PMID: 31390291).
Control Peptide
Dynamin 2 Protein (NBP2-47477PEP)
Publications
Read Publications using
NBP2-47477 in the following applications:

  • 2 publications
  • KO
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Dynamin 2 Antibody

  • CMTDI1CMT2M
  • CMTDIBdynamin II
  • DI-CMTB
  • DNM2
  • DYN2
  • DYN2EC 3.6.5.5
  • Dynamin 2
  • dynamin-2
  • DYNII

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dynamin 2 Antibody (NBP2-47477)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, KO, WB.


Filter By Application
ICC/IF
(2)
KO
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dynamin 2 Antibody (NBP2-47477) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Dynamin 2 Antibody (NBP2-47477)

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Bioinformatics

Gene Symbol DNM2