EPS15R Antibody

Western Blot: EPS15R Antibody [NB100-88149] - EPS15 depletion stabilizes ESCRT components HRS, STAM and TSG101. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/13841) licensed under a CC-BY license.

Western Blot: EPS15R Antibody [NB100-88149] - Detection of Human EPS15L1 on HeLa whole cell lysate using NB100-88149. EPS15L1 was also immunoprecipitated by another rabbit anti-EPS15L1 antibody.

Western Blot: EPS15R Antibody [NB100-88149] - EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(D) HeLa cells stably expressing GFP-tagged wild-type EPS15, EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR & for control tubulin (TUB) analyzed by immunoblotting with specific antibodies. Experiments quantified in Fiji & the EPS15 levels plotted as fold-increase compared to controls. Data mean ± SEM, N = 4. *p≤0.05. Note that SPOPL depletion does not further increase the levels of both EPS15 mutants. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: EPS15R Antibody [NB100-88149] - EPS15 is targeted via a SPOP/SPOPL binding consensus motif. (C) Purified SPOPL was incubated as indicated with GST-tagged wild-type EPS15 or GST-EPS15 mutants, where the predicted SPOPL binding motifs have been mutated individually (GST-EPS15S605-S607A & EPS15S744-S746A, respectively), pulled down with glutathione sepharose (IP [GST]) & bound proteins analyzed by Coomassie blue staining (upper panel) & immunoblotting (lower panels). Note that SPOPL readily binds to GST-EPS15 & GST-EPS15S605-S607A, but this interaction is strongly reduced with GST-EPS15S744-S746A mutant. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EPS15R Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 814 and 864 of human epidermal growth factor receptor pathway substrate 15-like 1 using the numbering given in entry NP_067058.1 (GeneID 58513).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPS15L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  EPS15R Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EPS15R Antibody

• epidermal growth factor receptor pathway substrate 15-like 1
• epidermal growth factor receptor substrate 15-like 1
• epidermal growth factor receptor substrate EPS15R
• EPS15R
• Eps15-related protein

Background

EPS15L1 (epidermal growth factor receptor substrate 15-like 1), also known as EPS15R (EPS15-related), is structurally related to the EPS15 protein, a protein involved in the receptor-mediated endocytosis of the EGF receptor. EPS15L1 and EPS15 both contain an EH (Eps15 homology) domain, a protein-protein interaction domain found in several proteins that participate in endocytosis, vesicle transport, and signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EPS15R Antibody (NB100-88149)(1)

Publication using NB100-88149

• Gschweitl M, Ulbricht A, Barnes CA et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. Elife. 2016-04-22 [PMID: 27008177] (WB, Human)

Bioinformatics

• Gene Symbol: EPS15L1
• Entrez:
  • 58513(Human)
• Uniprot:
  • NP_067058.1(Human)