epithelial Sodium Channel alpha Antibody - BSA Free

Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] -Mouse kidney.ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [N3C2], Internal (NBP2-16353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ENaC Alpha antibody [N3C2], Internal detects ENaC Alpha protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal (NBP2-16353) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

epithelial Sodium Channel alpha Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit epithelial Sodium Channel alpha Antibody - BSA Free (NBP2-16353) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human epithelial Sodium Channel alpha. The exact sequence is proprietary.
• Localization: Apical cell membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCNN1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (85%), Rat (85%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for epithelial Sodium Channel alpha Antibody - BSA Free

• alpha ENaC-2
• Alpha-ENaC
• Alpha-NaCH
• amiloride-sensitive epithelial sodium channel alpha subunit
• amiloride-sensitive sodium channel subunit alpha 2
• amiloride-sensitive sodium channel subunit alpha
• BESC2
• ENaCA
• ENaCalpha
• Epithelial Na(+) channel subunit alpha
• epithelial Sodium Channel alpha
• FLJ21883
• nasal epithelial sodium channel alpha subunit
• Nonvoltage-gated sodium channel 1 subunit alpha
• SCNEA
• SCNN1
• SCNN1A
• sodium channel, nonvoltage-gated 1 alpha

Background

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SCNN1A
• Uniprot:
  • P37088(Human)