epithelial Sodium Channel beta Antibody

Western Blot: epithelial Sodium Channel beta Antibody [NBP3-18257] - Western blot analysis of Mouse mpkCCD cell lysates showing detection of epithelial Sodium Channel beta protein using Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257). Primary Antibody: Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257) at 1:1000.

Immunohistochemistry: epithelial Sodium Channel beta Antibody [NBP3-18257] - Immunohistochemistry analysis using Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western Blot: epithelial Sodium Channel beta Antibody [NBP3-18257] - Western blot analysis of Rat kidney tissue lysates showing detection of epithelial Sodium Channel beta protein using Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257). Primary Antibody: Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257) at 1:1000.

Western Blot: epithelial Sodium Channel beta Antibody [NBP3-18257] - Western blot analysis of Mouse kidney cortex showing detection of epithelial Sodium Channel beta protein using Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257). Primary Antibody: Rabbit Anti-epithelial Sodium Channel beta Polyclonal Antibody (NBP3-18257) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).

• Reactivity: Hu, Mu, Rt, Ha, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

epithelial Sodium Channel beta Antibody Summary

• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
• Localization: Apical Cell Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCNN1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
• Application Notes: Optimal dilutions for assays should be determined by the user.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for epithelial Sodium Channel beta Antibody

• amiloride-sensitive sodium channel subunit beta 1
• amiloride-sensitive sodium channel subunit beta
• BESC1
• Beta-ENaC
• Beta-NaCH
• ENaCB
• ENaCbeta
• Epithelial Na(+) channel subunit beta
• epithelial Sodium Channel beta
• epithelial sodium channel beta-2 subunit
• epithelial sodium channel beta-3 subunit
• nasal epithelial sodium channel beta subunit
• Nonvoltage-gated sodium channel 1 subunit beta
• SCNEB
• SCNN1B
• sodium channel, nonvoltage-gated 1, beta

Background

SCNN1B is a subunit of the epithelial sodium channel, ENaC. ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Mutations in beta or gamma subunit of the epithelial sodium channel (ENaC) have been found to cause a hereditary form of human hypertension, Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCNN1B